藤黄酸作用后小鼠血清代谢组学研究

Serum Metabolomics Study in Gambogic Acid Treated Mice

目的研究藤黄酸对小鼠血清代谢稳态的影响,发掘其体内效应分子。方法11只雌性小鼠随机分为两组,分别尾静脉注射藤黄酸(10 mg·kg^-1)和空白溶液。整合运用液相色谱-质谱(LC/MS)和气相色谱-质谱(GC/MS)技术全面表征各组小鼠血清代谢指纹谱,利用偏最小二乘-判别分析(PLS-DA)和(非)参数检验进行模式识别和统计分析,筛选、鉴定差异代谢物,聚焦相关代谢通路。结果与对照组比较,藤黄酸组小鼠血清中苏氨酸、丝氨酸、谷氨酸等14个物质含量显著上升,PC、LysoPE等7个物质含量显著下降,涉及氨基酸代谢、脂肪酸代谢、三羧酸循环等通路。结论藤黄酸能显著影响机体的氨基酸代谢、磷脂代谢以及能量代谢,提示其发挥体内作用可能与之有关。该研究结果可为深入揭示藤黄酸的作用机制提供一定参考。

Objective To study the effect of gambogic acid on serum metabolic homeostasis in mice,and to explore its effective molecules in vivo.Methods Eleven female mice were randomly divided into two groups,and they were injected with gambogic acid(10 mg/kg)and blank solution via tail veins,respectively.Liquid chromatography-mass spectrometry(LC/MS)and gas chromatography-mass spectrometry(GC/MS)were used as the integrated approach for the characterization of the mice serum metabolic fingerprints in two groups.Partial least squares-discriminant analysis(PLS-DA)and(non-)parametric tests were performed for pattern recognition and statistical analysis.Differential metabolites were screened and identified,and then the related metabolic pathways were focused.Results Compared with control group,the serum levels of 14 metabolites,such as threonine,serine and glutamic acid,were increased significantly in the gambogic acid treated group,while the levels of 7 metabolites such as PC and LysoPE were decreased significantly,involving amino acid metabolism,fatty acid metabolism,tricarboxylic acid cycle and other pathways.Conclusion Gambogic acid can significantly affect the amino acid metabolism,phospholipid metabolism and energy metabolism,suggesting that its effects may be related to these pathways.This study provides a reference for further revealing the mechanism of action of gambogic acid.