降氮煎剂对慢性肾衰大鼠肾功能的作用及其对尿酸的影响

Effects of Nitrogen-reducing Decoction on Renal Function and Levels of UA and Renal Tissue UTA1 in Rats with Chronic Renal Failure

目的研究降氮煎剂对腺嘌呤所致慢性肾衰竭大鼠模型的肾功能的作用及其对尿酸的影响。方法除空白组外,其他大鼠皆用腺嘌呤灌胃法来复制慢性肾衰竭的模型。将尿毒清作为对照组,按照体重层次将造模大鼠随机分为模型组,尿毒清组(给予尿毒清颗粒)及实验组(给予降氮煎剂),治疗14 d后,测试每组大鼠血肌酐(Scr)、尿素氮(BUN)、尿酸(UA)及肾组织URAT1表达的水平。结果①实验组SCR、BUN、UA明显低于尿毒清组及模型组(P<0.05);②实验组肾组织URAT1蛋白表达明显低于尿毒清组及模型组(P<0.05),而尿毒清组与模型组之间无显著差异(P>0.05)。结论降氮煎剂可以显著降低Scr、BUN、UA及肾组织URAT1水平,具有改善肾功能及独立降低尿酸的作用。

Objective:To study the effects of Nitrogen-reducing Decoction on the renal function and the levels of UA and renal tissue URAT1 in rats with adenine-induced chronic renal failure.Methods:Except the rats in the blank group,other rats were given adenine gavage to replicate the chronic renal failure model.By using Niaoduqing as a control group,the model rats were randomly sub-divided into model groups according to their weight.The Niaoduqing group(administered with Niaoduqing granules)and the experimental group(administered with nitrogen-reducing decoction)was both treated for 14 days.The levels of rat serum creatinine(SCR),blood urea nitrogen(BUN),uric acid(UA)and renal tissue URAT1 expression in each group were detected.Results:1)The SCR,BUN,and UA of the experimental group were significantly lower than those of the Niaoduqing group and the model group(P<0.05).2)The expression of URAT1 protein in the kidney tissue of the experimental group was significantly lower than that of the Niaoduqing group and the model group(P<0.05),but there was no significant difference between the Niaoduqing group and the model group(P>0.05).Conclusion:Nitrogen-reducing decoction can significantly reduce the levels of SCR,BUN and UA and has the effect of improving renal function,and has independent effects of reducing uric acid.