高压离子色谱法对火腿肠中亚硝酸盐的检测

Detection of Nitrite in Ham Intestine by High Pressure Ion Chromatography

为建立高压离子色谱法测定火腿肠中亚硝酸盐含量的方法,将样品经过超声和水浴提取后,经过C18小柱、Ag柱、Na柱和0.22μm过滤柱的净化,以氢氧化钾KOH为淋洗液,经Dionex IonPac AS11-HC-4μm(4×250 mm)阴离子柱分离,采用Dionex ADRS 600(4 mm)阴离子抑制器,电导检测器进行检测,标准曲线法定量。结果表明亚硝酸根离子在0.02~0.20mg/L范围内线性关系良好,相关系数为0.9999,样品中亚硝酸盐的加标回收率96.6%,相对标准偏差3.18%。结果表明,本方法科学、可靠,且因为采用了高压离子色谱仪,检验过程更加的高效和快速。

In order to establish a method for the determination of nitrite content in ham sausage by high pressure ion chromatography, the sample was subjected to ultrasonic water bath extraction, and then purified through a C18 column, an Ag column, a Na column, and a 0.22 μm filter column. As the eluent, it was separated by Dionex IonPac AS11-HC-4 μm(4×250 mm) anion column. Dionex ADRS 600(4 mm) anion suppressor and conductivity detector were used for detection, and the standard curve method was used for quantification. The results showed that the nitrite ion had a good linear relationship in the range of 0.02~0.20 mg/L, with a correlation coefficient of 0.9999. The spiked recovery of nitrite in the sample was 96.6 %, and the relative standard deviation was 3.18 %. The results show that the method is scientific and reliable, and because of the use of high-pressure ion chromatograph, the test process is more efficient and fast.