西瓜SSR标记中不同类型样品的DNA提取分析

DNA extraction from different types of watermelon samples in SSR markers

为实现西瓜品种纯度的室内快速检测,笔者对西瓜的真叶、子叶、种芽和种子进行了DNA提取,比较了不同类型样品提取DNA的质量与浓度差异,并研究了从不同类型样品中提取的DNA对于SSR-PCR的影响.结果表明,不同类型样品中提取的DNA浓度与质量的大小排序均为:真叶>子叶>种芽>种仁,其DNA浓度之间具有显著性差异.将上述材料中提取的DNA作为模板进行了SSR-PCR扩增分析,发现以上4组DNA模板均能扩增出清晰可见的目标条带,表明从种仁中可直接提取DNA进行SSR分子标记纯度检测.因此在室内用SSR分子标记检测品种纯度时可省略催芽育苗阶段,从而提升检测效率,节省时间.

In order to achieve rapid indoor identification of variety purity,in this study,the DNAs from true leaf,cotyledon,seedlings and seed of watermelon were extracted respectively,and the quality and concentration of them were compared,their influence for SSR-PCR were researched.The results showed that the concentration and quality of the extracted DNA were ranked as follows:true leaf>cotyledon>seedlings>seed,and the DNA concentration was significantly different.The DNAs extracted from the above materials was used as templates for SSR-PCR analysis respectively.They can all achieved the target band,indicating that the DNA extracted from the seed can be also used for SSR markers.Therefore,in the purity identification of watermelon cultivar by using SSR method,the germination and seedling stage can be omited,and the efficiency can be improved greatly and much time will be saved.