不同产地金银花的红外光谱鉴定

Identification of Lonicera japonica Producing Area by Infrared Spectrum

为不同产地金银花药材的快速、准确鉴定提供技术支撑,采用红外光谱指纹图谱的一阶、二阶导数图谱,鉴定云南、山东、安徽、湖北及江苏5个产地的10份金银花药材。结果表明:5个产地的金银花红外指纹原始谱图整体上无太大差异且振动峰位置基本相似,无法区分产地;一阶谱图中,湖北产金银花的吸收峰3411 cm^-1、1632 cm^-1、1378 cm^-1、1259 cm^-1、1047 cm^-1、804 cm^-1,江苏产金银花的吸收峰510 cm^-1、1410 cm^-1、827 cm^-1,均可将其与其余产地金银花区分开;二阶谱图中,安徽产金银花的吸收峰2800 cm^-1、2900 cm^-1和2300 cm^-1,云南产金银花吸收峰3668 cm^-1,湖北、江苏产金银花的吸收峰600 cm^-1、700 cm^-1、1700 cm^-1、2300 cm^-1、3600 cm^-1,均可与其余产地的样品进行区分。红外光谱的一阶、二阶导数光谱各个区段振动吸收峰的峰强、峰位及峰形可将5个产地的金银花区分开。

Ten Lonicera japonica samples from five producing areas of Yunnan,Shandong,Anhui,Hubei and Jiangsu are identified by first and second derivative diagram of infrared spectrum fingerprint to provide the technological support for rapid and accurate identification of L.japonica producing area.Result:There is no obvious difference in original spectra of infrared spectrum fingerprint among five producing areas of L.japonica and the position of their vibration peaks is basically similar,which indicates that the original spectra of infrared spectrum fingerprint cannot be used to distinguish the producing area of L.japonica.The first derivative spectra shows that six absorption peaks(3411 cm^-1,1632 cm^-1,1378 cm^-1,1259 cm^-1,1047 cm^-1 and 804 cm^-1)of L.japonica from Hubei can be used to distinguish L.japonica from other producing areas but three absorption peaks(510 cm^-1,1410 cm^-1 and 827 cm^-1)of L.japonica from Jiangsu can be used to distinguish L.japonica from other producing areas.The second derivative spectra shows that three absorption peaks(2800 cm^-1,2900 cm^-1 and 2300 cm^-1)of L.japonica from Anhui can be used to distinguish L.japonica from other producing areas and one absorption peak(3668 cm^-1)of L.japonica from Yunnan can be used to distinguish L.japonica from other producing areas but five absorption peaks(600 cm^-1,700 cm^-1,1700 cm^-1,2300 cm^-1 and 3600 cm^-1)of L.japonica from Hubei and Jiangsu can be used to distinguish L.japonica from other producing areas.The peak intensity,peak position and peak shape of vibration absorption peaks in different section of first and second derivative spectra of infrared spectrum can be used to distinguish five producing areas of L.japonica.