“金花散茶”及“金花菌粉”对被动吸烟小鼠肺组织JAK2/STAT3炎性及磷酸化蛋白表达的影响

Effects of‘Eurotium cristatum Loose Tea’and‘Eurotium cristatum Powder’on the Expressions of JAK2/STAT3 Inflammation and Phosphorylated Proteins in Lung Tissue of Passive Smoking Mice

为探究"金花散茶"及其"金花菌粉"对被动吸烟(Cigarette smoking environment,CSE)小鼠肺组织受损的预防及修复机制,建立C57BL/6小鼠CSE模型,以600 mg·kg-1剂量的金花散茶茶汤(Eurotium cristatum tea extract,ECTE)及金花菌粉浸提液(Eurotium cristatum powder extract,ECPE)进行灌喂处理。与CSE模型组相比,小鼠灌喂ECPE和ECTE后,肺组织病理学切片显示其可保护小鼠肺组织形态结构完整;酶联免疫分析显示,灌喂ECPE和ECTE可显著抑制小鼠血清IL-6、IL-8、IL-1β、IFN-γ和TNF-α表达量上调;Western blot结果表明,灌喂ECPE和ECTE对小鼠肺组织p-JAK2、p-STAT3、p-JAK2/JAK2、p-STAT3/STAT3高表达起到抑制作用。以上研究结果表明,灌喂ECPE、ECTE对CSE肺受损小鼠具有明显保护作用,总体趋势为ECPE组优于ECTE组、预防组优于治疗组。

In order to investigate the prevention and recovery mechanism of’Eurotium cristatum Loose Tea’and its’Eurotium cristatum powder’on mouse lung tissues which were injured by passive smoking,passive cigarette smoking environment(CSE)model on SPF C57 BL/6 female mice were established.Mice were fed by 600 mg·kg-1 Eurotium cristatum tea extract(ECTE)and Eurotium cristatum powder extract(ECPE).Comparing with the CSE model mice,the morphology integrity of lung tissue in passive smoking mice feeding with ECPE and ECTE were significantly protected by observing the pathological slice of lung tissue.The up-regulation levels of IL-6,IL-8,IL-1β,IFN-γand TNF-αin the serum of mice were inhibited by ELISA analysis.Western blot results show that the expression levels of p-JAK2,p-STAT3,p-JAK2/JAK2,p-STAT3/STAT3 in lung tissues of passive smoking mice fed with ECPE and ECTE were inhibited.These results reveal the prominent protective roles of ECPE and ECTE in the lung injury of passive smoking mice.As a whole,ECPE feeding groups were superior to ECTE feeding groups,while prevention groups were better than treatment groups.