HPLC法测定冬虫夏草胶囊中腺苷的含量

Determination of Adenosine Content in Dongxia Chongcao Capsules by HPLC

目的:建立院内制剂冬虫夏草胶囊中腺苷含量的测定方法,为提高该制剂的质量标准提供依据。方法:采用高效液相色谱法,色谱柱为Hibar-C18柱(4.6 mm×150 mm,5μm);流动相为pH值6.5的磷酸盐缓冲液-甲醇(85∶15);检测波长为260 nm;柱温30℃;流速为1.0 mL/min;进样量为10μL。结果:腺苷在0.0327~0.1635μg范围内,线性关系良好(R^2=0.9994);加样回收率为99.8%~101.5%;测定3个批次的冬虫夏草胶囊中腺苷的平均含量分别为0.91 mg/粒,0.92 mg/粒,0.93 mg/粒,相对标准偏差(RSD)分别为0.44%、1.15%、2.06%。结论:该方法简便,准确,重现性好,灵敏度高,可作为冬虫夏草胶囊质量控制指标;所测3批冬虫夏草胶囊中腺苷的含量相近,质量可靠,可用于临床治疗。

Objective:To develop an HPLC method for the determination of adenosine in Dongxia Chongcao Capsules and provide a basis for improving the quality standards of the preparation.Methods:HPLC was used and the analytical column was Hibar-C18(4.6 mm×150 mm,5μm).The mobile phase was phosphate buffer pH6.5-methanol(85∶15).The detection wavelength was 260 nm.The column temperature was 30℃.The flow was 1.0 mL/min.The injection volume is 10μl.Results:Adenosine had a good linear relationship(R^2=0.9994)in the 0.0327~0.1635μg range,and the sample recovery was 99.8%~101.5%.The average contents of adenosine in 3 batch slices of Chinese caterpillar fungus Capsules was 0.91 mg,0.92 mg,0.93 mg.The relative standard deviations(RSD)were 0.44%,1.15%,and 2.06%,respectively.Conclusion:The contents of adenosine in the 3 batches of Dongxia Chongcao Capsules are analogical.This method is simple,accurate and available for the quality control of Dongxia Chongcao Capsules.The 3 batches of Dongxia Chongcao Capsules have similar adenosine content and reliable quality,which can be used for clinical treatment.