益肾祛浊方对多囊卵巢综合征胰岛素抵抗模型大鼠激素受体表达的干预作用

Study on Intervention Effects of Yishen Quzhuo Formula on Hormone Receptor Expression in PCOS-IR Model Rats

目的:探索益肾祛浊方对多囊卵巢综合征胰岛素抵抗(PCOS-IR)模型大鼠激素及胰岛素受体表达的干预作用。方法:选取6~8周龄SPF级SD雌性大鼠60只随机分为空白组、模型组、益肾祛浊组、益肾组、西药组(达英-35联合二甲双胍),每组12只。空白组给予普通饲料喂养,其余各组均给予来曲唑灌胃联合高脂饲料喂养至第27天。然后给予不同药物灌胃2周,采用免疫荧光法检测各组大鼠雄激素受体(AR)、促黄体生成素受体(LHR)在卵巢、胃、肠组织的表达;并观察胰岛素受体(INSR)在卵巢的表达。结果:治疗14 d后发现,益肾祛浊方可以显著降低大鼠卵巢局部AR、LHR和INSR的表达,差异有统计学意义(P<0.05);且明显减少胃中AR及LHR的表达,差异有统计学意义(P<0.05);同时显著减少结肠组织LHR的表达,差异有统计学意义(P<0.05)。结论:益肾祛浊方可能通过降低AR、LHR表达而调整模型大鼠内分泌紊乱,又可通过降低INSR的表达,对PCOS-IR大鼠的代谢紊异常起到治疗作用。

Objective:To explore the intervention effects of Yishen Quzhuo Formula on the expression of hormones and insulin receptors in polycystic ovary syndrome model(PCOS-IR)rats.Methods:A total of 60 SPF level SD female rats aged 6-8 weeks were randomly divided into a blank group,a model group,a Yishen Quzhuo group,a Yishen Quzhuo group,and a Western medicine group(Diane-35 combined with metformin),with 12 rats in each group.The blank group was fed with normal diet,and the other groups were fed with letrozole by gavage combined with high-fat diet until the 27th day.Then,different drugs were fed by gavage for 2 weeks.The expression of androgen receptor(AR),luteinizing hormone receptor(LHR)in ovary,stomach and intestine and insulin receptor(INSR)in ovary were observed by immunofluorescence.Results:After 14 days of treatment,Yishen Quzhuo Formula could significantly reduce the expression of AR,LHR and INSR in part of ovary in rats(P<0.05),the expression of AR and LHR in stomach(P<0.05),and the expression of LHR in colon tissue(P<0.05).Conclusion:Yishen Quzhuo Formula may regulate endocrine disorders in model rats by lowering the expression of AR,LHR and further exert therapeutic effects on metabolic disorder of PCOS-IR rats by reducing the expression level of INSR.