广东省人群肺癌罹患风险与P73基因G4C14-to-A4T14多态性的相关性分析

Correlation analysis between P73 gene G4C14-to-A4T14 polymorphism and lung cancer risk in Guangdong population

目的研究旨在探讨P73基因G4C14-to-A4T14多态性与广东省人群肺癌罹患风险的相关性。方法采用HRM方法(高分辨率熔解曲线)对642例肺癌患者(包含450例NSCLC和SCLC192例)和354例正常对照者外周血中P73基因多态性进行了基因型分析。结果HRM基因分型结果显示,450例NSCLC患者中P73基因型分布如下:GC/GC 280例(62.22%),GC/AT 155例(34.44%),AT/AT 15例(3.33%)。192例SCLC患者中P73基因型:GC/GC 118例(61.46%),GC/AT 67例(34.90%),AT/AT 7例(3.65%)。354例正常对照组P73基因型:GC/GC 192例(53.10%),GC/AT 136例(38.42%),AT/AT 26例(8.40%)。AT/AT纯合基因型携带者与非携带者相比,显著降低NSCLC(OR=0.393;95%CI:0.037~0.873;P=0.001)和SCLC(OR=0.428;95%CI:0.050~0.880;P<0.010)的风险。结论研究结果表明P73 G4C14-A4T14多态性可能是广东省肺癌人群中易感性的一个修饰因子,P73基因中GC含量的增加会增加罹患肺癌的风险。

Objective To analyze the correlation between P73 gene G4C14-to-A4T14 double nucleotide polymorphism and the risk of lung cancer in Guangdong population.Methods Genotype analysis of P73 gene polymorphism in peripheral blood of 642 patients with lung cancers(including 450 NSCLC patients and 192 SCLC patients)and 354 normal controls was performed with HRM method(high-resolution fusion curve).Results HRM genotyping results showed that the distribution of P73 genotypes in 450 NSCLC patients was as follows:GC/GC 280(62.3%),GC/AT 155(34.4%),and AT/AT 15(3.3%).P73 genotypes in 192 SCLC patients were 118 GC/GC(61.5%),67 GC/AT(34.9%)and 7 AT/AT(3.6%).The P73 genotypes of 354 normal controls were 192 GC/GC(53.1%),136 GC/AT(38.5%),and 26 AT/AT(8.4%).AT/AT homozygous genotypes significantly reduced the risk of NSCLC(OR=0.393;95%CI:0.037-0.873;P=0.001)and SCLC(OR=0.428;95%CI:0.050-0.880;P<0.001)compared with non-carriers.Conclusion The results of the present study indicated that the polymorphism of P73 G4C14-A4T14 may be a modification factor for the susceptibility of lung cancer in Guangdong province,and the increased GC content in the P73 gene may increase the risk of lung cancer.