鲜食番茄品种遗传多样性分析及指纹图谱构建

Genetic Diversity Analysis and Fingerprinting Construction of Fresh Tomato Varieties

选取市场上广泛使用和拟进入市场的75份番茄材料,利用栽培种间多态性丰富的48对InDel标记进行遗传多样性分析,并选取其中24对标记进行指纹图谱构建。结果表明,48对InDel标记中,45对呈现较好的多态性;75份番茄材料的遗传距离为0.16~0.91,平均遗传距离为0.52;聚类分析将75份材料分为5个类群,其中第Ⅰ、Ⅳ和Ⅴ类群以国内品种为主,第Ⅱ和Ⅲ类群主要集中了国外公司的一些品种;选取24对覆盖全基因组的InDel标记进行指纹图谱构建,基本可以将75份番茄材料进行识别。

This paper selected 75 tomato varieties widely used by market or planed to enter market;and analyzed the genetic diversity of these varieties showing rich polymorphic among tomato cultivars using 48 InDel markers.Then,24 markers were chosen to construct fingerprinting of these varieties.The result indicated that 45 out of 48 primers showed better polymorphism.The genetic distance of 75 tomato F1 hybrids were 0.16-0.91 with an average around 0.52.Seventy-five F1 hybrids were divided into 5 groups.Among them groupⅠ,ⅣandⅤwere mainly from the national company,whereas groupⅡandⅢwere mainly from the international companies.Twenty-four InDel markers covering 12 chromosomes were used to construct fingerprinting of these varieties.They could basicly recognize these 75 tomato varieties.