类风湿关节炎患者舌苔菌群微生态研究

Microecology of tongue coating flora in patients with rheumatoid arthritis

目的从舌苔菌群微生态上研究类风湿关节炎患者疾病进展与菌群的关系。方法使用口腔咽拭子刮取活动期(OP组)、缓解期(OR组)及健康对照组(OHC)各10例的舌苔,使用口腔拭子基因组DNA快速提取试剂盒提取总DNA,进行16S rRNA扩增并进行Illumina MiSeq高通量测序,对测序结果进行微生态分析。结果3组样本在菌群的多样性上差异无统计学意义(P>0.05);3组样本有着各自独有的菌属,其中OP组7个菌属,OR组4个菌属,OHC组10个菌属为各组独有;3组样本的优势菌群组成相同,但是在菌群构成中差异具有统计学意义,主要在厚壁菌门、放线菌门、Episilonbacteraeota菌门、变形菌门、奈瑟菌属、放线菌属、弯曲杆菌属和卟啉单胞菌属上(P均<0.05)。进行Beta多样性分析,通过PCA分析以及层级聚类分析,OP组和OHC组能够很好地聚集到一起,差异具有统计学意义。结论类风湿关节炎患者舌苔菌群与健康人群存在差异,其中主要表现在放线菌属和弯曲杆菌属丰度增加,奈瑟菌属和卟啉单胞菌属丰度减少。

Objective To investigate the relationship between disease progression and microecology of tongue coating flora in patients with rheumatoid arthritis(RA).Methods A total of 30 tongue coating flora swabs were collected from 20 RA patients,including 10 in the active stage(OP group)and 10 in the remission stage(OR group),and 10 healthy individuals(OHC group).The total DNA extracted by rapid oral swab genomic DNA extraction kit was amplified with 16S rRNA and sequenced with high throughput Illumina MiSeq.The results were analyzed by microecology.Results There was no statistical difference in the diversity of 3 groups of samples(P>0.05).All the 3 groups of samples had their own unique genera,including 7 genera in OP group,4 genera in OR group,and 10 genera in OHC group;The samples from 3 groups had the same composition of dominant flora,but obvious difference was seen in the composition of flora,mainly in p_Firmicutes,p_Actinobacteria,p_Episilonbacteraeota,p_Proteobacteria,g_Neisseria,g_Actinomycetes,g_Campylobacter,and g_Porphyromonas(all P<0.05).Through PCA analysis and hierarchical cluster analysis,OP group and OHC group can gather together well with significant differences.Conclusion There are differences between the flora of tongue coating in RA patients and healthy individuals.The abundances of Actinomycetes and Campylobacter are increased,while those of Neisseria and Porphyromonas are decreased.