miR-1-3p在食蟹猴心肌损伤中表达水平及其与CK-MB、cTnI和NT-proBNP的相关性

Expression of miR-1-3p in myocardial injury cynomolgus monkey and its correlation with CK-MB,cTnI and NT-proBNP

目的探讨循环miR-1-3p在多柔比星诱导食蟹猴心肌损伤模型中的表达水平和诊断价值。方法选取3~5岁普通级雄性食蟹猴17只,随机分为对照组(7只)和实验组(10只),静脉注射盐酸多柔比星注射液(2.5 mg/kg)4周,收集外周血浆标本和心脏组织标本,应用液相芯片技术测定肌酸激酶同工酶(CK-MB)、肌钙蛋白I(cTnI)和NT-proBNP表达水平,且应用实时定量PCR技术测定miR-1-3p表达的水平,并进行相关性分析。结果成功建立药物致食蟹猴心肌损伤模型;两组CK-MB、cTnI和NT-proBNP水平比较差异有统计学意义(P<0.05);实验组miR-1-3p的表达水平在给药4周后较给药前和对照组相比明显升高(P<0.05);miR-1-3p的表达水平与CK-MB、cTnI和NT-proBNP改变呈现了相同的升高趋势,且二者之间具有明显相关性。结论miR-1-3p可作为药物引起心肌损伤的生物学诊断标志物之一。

Objective To investigate the expression level and diagnostic value of circulating miR-1-3 p in doxorubicin-induced myocardial injury model of cynomolgus monkey.Methods Totally 17 normal male cynomolgus monkeys aged 3~5 years were randomly divided into control group(n=7)and experimental group(n=10).Doxorubicin hydrochloride injection(2.5 mg/kg)was injected intravenously for 4 weeks.The peripheral plasma samples and heart tissue samples were collected.The expression levels of CK-MB,cTnI and NT-proBNP were measured by liquid chip technology,the expression level of miR-1-3 p was determined by real-time quantitative PCR,and the correlation was analyzed.Results The myocardial injury model of cynomolgus monkey was established successfully.There were significant differences in the levels of CK-MB,cTnI and NT-proBNP between the two groups(P<0.05).The expression level of miR-1-3 p in experimental group was significantly higher than that in control group after 4 weeks of administration(P<0.05).The expression level of miR-1-3 p showed the same increasing trend with the changes of CK-MB,cTnI and NT-proBNP,and there was a significant correlation between them.Conclusion miR-1-3 p can be used as one of the biomarkers of myocardial injury caused by drugs.