CD44和RHAMM在胃肠肿瘤中的表达及其临床意义

Detection of CD44 and RHAMM Expression and Prognosis in Gastrointestinal Tumors by Real-time Fluorescence Quantitative PCR

目的研究实时荧光定量PCR检测CD44和RHAMM在胃肠肿瘤中的表达及其临床意义。方法随机选取胃肠肿瘤患者95例,将肿瘤组织及其周边5cm以上的正常组织取出来,保存在液氮中。进行总RNA抽提与cDNA合成、逆转录-PCR、CD44、RHAMM GAPDH质粒制备、实时PCR。结果实时荧光定量PCR检测CD44、RHAMM在胃肠肿瘤组织中的表达均显著高于正常组织(P<0.05)。CD44和RHAMM在胃肠肿瘤的表达显著相关(P<0.05),其中肠道、有淋巴结转移CD44和RHAMM均呈显著的正相关关系(P<0.05),但胃部、无淋巴结转移CD44和RHAMM均无相关性(P>0.05)。结论实时荧光定量PCR检测CD44和RHAMM在胃肠肿瘤组织中的表达均高于正常组织,且具有相关性,能够对肿瘤淋巴结转移情况进行判定参考。

Objective To study the expression and prognosis of CD44 and RHAMM in gastrointestinal tumors by real-time fluorescence quantitative PCR.Methods 95 patients with gastrointestinal tumors were randomly selected.The normal tissues above 5 cm around the tumors were taken out and stored in liquid nitrogen.Total RNA extraction and DNA synthesis,reverse transcription-PCR,CD44,RHAMM GAPDH plasmid preparation,real-time PCR were carried out.Results The expression of CD44 in gastrointestinal tumors was significantly higher than that in normal tissues by real-time fluorescence quantitative PCR(P<0.05).The expression of RHAMM in gastrointestinal tumors was significantly higher than that in normal tissues by real-time fluorescence quantitative PCR(P<0.05).CD44 and RHAMM were positively correlated in intestinal tract,lymph node metastasis(P<0.05),but not in stomach and non-lymph node metastasis(P>0.05).Conclusion The expression of CD44 and RHAMM in gastrointestinal cancer tissues detected by real-time fluorescence quantitative PCR is higher than that in normal tissues,and has correlation.It can provide effective basis for clinical diagnosis of lymph node metastasis.