蓖麻GST的基因克隆及生物信息学分析

Gene Cloning and Bioinformatics Analysis of Castor GST

谷胱甘肽S-转移酶(glutathione S-transferase,GST)是谷胱甘肽结合反应的关键酶,催化谷胱甘肽结合反应的起始步骤,主要存在于胞液中,有多种形式,其在病理学上有一定的重要性。本研究通过克隆GST基因以及对其进行相关的生物信息学分析。利用聚合酶链式反应(polymerase chain reaction,PCR)技术扩增该基因,经分析该基因可编码219个氨基酸,并且通过相关分析得到这219个氨基酸组成的蛋白为稳定的亲水性蛋白,有一个明显的区段存在信号肽,在该蛋白中α-螺旋和无规则卷曲是主要的二级结构方式,根据序列同源比对,蓖麻、麻疯树和榴莲聚类到一起,说明相对于其他物种,它们的同源性较高。这些分析结果为蓖麻谷胱甘肽S-转移酶生物合成及其功能的进一步研究提供一定的理论与实践依据。

Glutathione S-transferase(GST)is the key enzyme of glutathione binding reaction.It catalyzes the initial steps of glutathione binding reaction.It mainly exists in the cytosol and has many forms.It has certain pathological importance.In this study,GST gene was cloned and analyzed by bioinformatics.Polymerase chain reaction(PCR)was used to amplify the gene.The gene can encode 219 amino acids.The protein composed of 219 amino acids is a stable hydrophilic protein.There is an obvious signal peptide in the protein,in which alpha-helix and random coil are the main secondary structure.According to sequence homology alignment,castor,Jatropha curcas and durian clustered together,indicating that they had higher homology than other species.These results provide a theoretical and practical basis for further research on biosynthesis and function of castor glutathione S-transferase.