线粒体DNA含量测定在早期胚胎评估中的研究进展

Research progress of mitochondrial DNA content on early embryo assessment

线粒体是细胞质中含量最丰富的细胞器,为细胞内各种代谢提供能量,在卵母细胞减数分裂、受精和早期胚胎发育的过程中发挥着巨大的作用。卵母细胞线粒体DNA含量的高低与其后续的胚胎发育潜能密切相关。本文从无创性测定线粒体DNA含量角度出发,就颗粒细胞、卵裂球及滋养外胚层细胞和胚胎剩余培养基及囊胚腔液中线粒体DNA含量测定在胚胎评估上的研究做一综述,并探讨其在早期胚胎评估中的应用前景。

Mitochondria are the most abundant organelles in cytoplasm,which provide energy for various metabolism in cells.Mitochondria play an important role in oocyte meiosis,fertilization and early embryonic development.The content of mitochondrial DNA in oocytes is closely related to the development potential of subsequent embryos.From the viewpoint of non-invasive assessment of mitochondria DNA content in this context,here,we present an objective review of current research progress of mitochondria DNA content detection on granular cells,embryo cleavage cells/trophectoderm and spent culture media/blastocoele fluid,to explore its application prospect on early embryo development.