MiR-122与miR-33a在2型糖尿病合并冠心病患者中的表达

Expressions of miR-122 and miR-33a in patients with type 2 diabetes complicated with coronary artery disease

目的 探讨2型糖尿病(T2DM)合并冠状动脉粥样硬化性心脏病(CAD)患者血清miR-122和miR-33a表达的改变及其意义.方法 收集2016年7月至2018年7月收治的2型糖尿病患者32例(T2DM组)、冠心病患者32例(CAD组)、2型糖尿病合并冠心病患者32例(T2DM+CAD组)的临床资料,另选择同期体检的健康者32例(对照组).经分离提纯血浆总microRNA,采用实时定量PCR法检测miR122和miR33a的表达量.结果 与对照组相比,T2DM组、CAD组和T2DM+CAD组血浆miR122mRNA表达量明显增加(P<0.05);与T2DM组相比,CAD组和T2DM+CAD组miR-122mRNA表达量明显增加(P<0.05).miR-122对CAD组和T2DM+CAD组的ROC曲线下面积分别为0.72±0.05与0.77±0.04.各组间miR-33a表达水平无明显差异(P>0.05).结论 血浆miR-122是2型糖尿病合并冠心病较好的辅助诊断指标.

Objective To investigate the expressions and diagnostic significance of serum miR-122 and miR-33a in pa-tients with type 2 diabetes mellitus(T2DM)complicated with coronary artery heart disease(CAD).Methods Fast bloods samples were collected from 32 healthy volunteers(NC group),32 T2DM patients(T2DM group),32 CAD pa-tients(CAD group)and 32 patients with T2DM and CAD(T2DM+CAD group).The expressions of miR-122 and miR-33a were detected with real-time quantitative PCR.Specificity and sensitivity of the association between miR-122 in T2DM and CAD were evaluated with receiver operating characteristic(ROC)and area under the curve(AUC).Results Com-pared with the NC group,the other three groups had significantly higher expressions of miR-122(P<0.05).Compared with T2DM group,the CAD and T2DM+CAD groups had significantly higher expression of miR-122(P<0.05).The AUC of miR-122 in CAD group and T2DM+CAD group was 0.72±0.05 and 0.77±0.04,respectively.There were no sig-nificant differences in the miR-33a expression among the four groups(P>0.05).Conclusion Serum miR-122 can serve as a good diagnostic marker for T2DM complicated with CAD.