常温条件下厌氧氨氧化污泥储存及激活

Storage and activation of Anaerobic ammonium oxidation sludge under room temperature conditions

在常温(20℃)且不补充营养液的条件下,在填充50%(体积比)蜂窝状填料的广口瓶中储存Anammox污泥30 d。储存后,细菌密度从3.635×10^11 mL^-1减少到3.012×10^11 mL^-1,AnAOB密度从2.338×10^11 mL^-1减少到1.223×10^11 mL^-1,表明储存后的Anammox污泥仍有一定比例的Anammox菌。激活过程HRT保持为1 d,通过逐渐提高底物浓度来提高进水氮负荷。初始进水NH4^+-N和NO2^--N质量浓度为50 mg/L,经15 d激活,其去除率分别达到81.36%、99.34%,表明Anammox成功启动。经过55 d,进水NH4^+-N和NO2^--N达到200 mg/L,去除率分别为83.52%、99.99%。

Anammox sludge is stored for 30 days in a jar filled with 50%honeycomb packing at room tempera-ture(20℃)without nutrient solution.After storage,the bacterial density decreases from 3.635×10^11 mL^-1 to 3.012×10^11 mL^-1,and the AnAOB density decreases from 2.338×10^11 mL^-1 to 1.223×10^11 mL^-1,which indicates that Anammox sludge still has a certain proportion of Anammox bacteria.The HRT remains for one day during the activation proce-ss,and the influent nitrogen load is increased by increasing the substrate concentration gradually.The initial influent NH4^+and NO2^--N is 50 mg/L.After 15 days of activation,the removal rates of NH4^+-N and NO2^--N reach 81.36%and 99.34%,respectively,when the initial influent NH4^+-N and NO2^--N are 50 mg/L,indicating that Anammox is succe-ssfully initiated.After 55 days,the influent NH4^+-N and NO2^--N reach 200 mg/L,the removal rates are 83.52%and 99.99%,respectively.