桑椹醇提物的化学成分研究

Chemical constituents of alcohol extract of Mori Fructus

目的研究桑椹Mori Fructus醇提物的化学成分。方法采用硅胶、Sephadex LH-20和ODS柱色谱法及半制备型高效液相色谱进行分离纯化,通过其理化性质和波谱数据对化合物结构进行鉴定。结果从桑椹醇提物中分离得到12个化合物,分别鉴定为桑脂素苷(1)、(7R,8S)-4,7,9,9′-tetrahydroxy-3,3′-dimethoxy-8-O-4′-neolignan-9′-O-β-D-glucopyranoside(2)、2-苯乙基-β-D-吡喃葡萄糖苷(3)、1′-O-苯乙基-β-D-呋喃芹菜糖基-(1→2)-β-D-吡喃葡萄糖苷(4)、苯甲醇-O-β-D-吡喃葡萄糖苷(5)、过氧麦角甾醇(6)、(24R)-6β-hydroxy-24-ethyl-cholest-4-en-3-one(7)、(22E)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol(8)、trans-(S)-(+)-脱落酸(9)、cis-(S)-(+)-脱落酸(10)、(S)-(+)-1-甲基-脱落-6-酸(11)、菜豆酸(12)。结论化合物1为未见文献报道的新化合物,化合物2、7~12为首次从该植物中分离得到。

Objective To investigate the chemical constituents of alcohol extract of Mori Fructus. Methods The chemical constituents were isolated and identified by chromatography on silica gel, Sephadex LH-20, ODS, and RP-HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral analyses. Results Twelve compounds were isolated from the alcohol extract of Mori Fructus, and identified as mullignanoside(1),(7 R,8 S)-4,7,9,9′-tetrahydroxy-3,3′-dimethoxy-8-O-4′-neolignan-9′-O-β-D-glucopyranoside(2), 2-phenylethyl-β-D-glucopyranoside(3), 1′-O-phenethyl-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(4), benzyl-O-β-D-glucopyranoside(5), ergosterol peroxide(6),(24 R)-6β-hydroxy-24-ethyl-cholest-4-en-3-one(7),(22 E)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol(8), trans-(S)-(+)-abscisic acid(9), cis-(S)-(+)-abscisic acid(10),(S)-(+)-1-methyl-abscisic-6-acid(11) and phaseic acid(12). Conclusion Compound 1 is a new compound named mullignanoside, and compounds 2, 7-12 are isolated from this plant for the first time.