丝穗金粟兰化学成分研究

Studies on chemical constituents of Chloranthus fortunei

目的对丝穗金粟兰Chloranthus fortunei的化学成分进行研究。方法利用多种色谱分离方法和波谱学鉴定方法对丝穗金粟兰中的化学成分进行了分离鉴定,并借助MTT法对其中得到的部分化合物进行了抗肿瘤活性的初步筛选。结果从丝穗金粟兰95%乙醇提取物中分离得到16个化合物,分别鉴定为迷迭香酸(1)、2′-羟基-4,3′,4′,6′-四甲氧基查耳酮(2)、卡瓦胡椒素A(3)、cycloshizukaol A(4)、白术内酯III(5)、4β-hydroxy-8,12-epoxyeudesma-7,11-diene-1,6-dione(6)、(8α)-6,8-dihydroxycadina-7(11),10(15)-dien-12-oic acidγ-lactone(7)、curcolonol(8)、11-hydroxyldrim-8,12-en-14-oic acid(9)、木栓酮(10)、异香草酸(11)、6β-hydroxystigmast-4-en-3-one(12)、3,4-二羟基苯甲酸(13)、莽草酸(14)、东莨菪苷(15)以及N-acetyltyramine 1-O-β-D-glucoside(16)。化合物4和5表现出微弱的细胞毒作用,半数抑制浓度(IC50)值在46~85μmol/L。结论化合物2、10、11、13~15为首次从金粟兰属植物中获得,化合物1~3、6~16为首次从丝穗金粟兰中分离得到。丝穗金粟兰中部分倍半萜显示出弱的抗肿瘤活性。

Objective To study the antitumor constituents from Chloranthus fortunei. Methods Various chromatographic techniques and spectroscopic methods were applied to investigate the chemical constituents from C. fortunei, and some of the compounds were screened for their antitumor activities by MTT method. Results Sixteen compounds were obtained from the whole plants of C. fortunei and identified as rosmarinic acid(1), 2′-hydroxy-4,3′,4′,6′-tetramethoxychalcone(2), flavokawain A(3), cycloshizukaol A(4), atractylenolide III(5), 4β-hydroxy-8,12-epoxyeudesma-7,11-diene-1,6-dione(6),(8α)-6,8-dihydroxycadina-7(11),10(15)-dien-12-oic acid γ-lactone(7), curcolonol(8), 11-hydroxyldrim-8,12-en-14-oic acid(9), friedelin(10), isovanillic acid(11), 6β-hydroxystigmast-4-en-3-one(12), 3,4-dihydroxybenzoic acid(13), shikimic acid(14), scopolin(15) and N-acetyltyramine 1-O-β-D-glucoside(16). Compounds 4 and 5 showed weak cytotoxicity with IC50 ranged from 46 to 85 μmol/L. Conclusion Compounds 2, 10, 11, and 13-15 are obtained from the genus Chloranthus for the first time and compounds 1-3 and 6-16 are isolated from C. fortunei for the first time. Some sesquiterpenoids from C. fortunei exhibited weak antitumor activities.