近红外光谱技术在金银花和山银花判别中的应用研究

Application of Near Infrared Spectroscopy in Identification of Lonicerae Japonicae Flos and Lonicerae Flos

目的:通过近红外光谱技术研究金银花和山银花在真伪鉴别中的应用。方法:本研究利用近红外光谱方法识别金银花和山银花的差异性,通过收集来自不同产地的101种金银花和山银花样品,采用近红外光谱选择5300~5550、6900~7500、8200~12000 cm^-1处光谱波段进行标准化预处理后,在主成分分析的聚类基础上通过SIMCA模式识别原理对金银花和山银花分别建立了类模型。结果:模型基本能正确识别金银花和山银花,所建立的方法可靠、快速简单,可作为金银花真伪判别的一种有效控制方法。结论:近红外光谱结合SIMCA模式识别在金银花和山银花分类识别具有可行性。

Objective:To study the application of Lonicerae Japonicae Flos and Lonicerae Flos in the identification of authenticity by near-infrared spectroscopy.Methods:The differences between Lonicerae Japonicae Flos and Lonicerae Flos were detected identified by near-infrared spectroscopy at 5300-5550,6900-7500,8200-12000 cm^-1,101 samples collected from different regions were detected,and after standardization preprocessing in the spectral band,based on the clustering of principal component analysis,a class model was established for Lonicerae Japonicae Flos and Lonicerae Flos by SIMCA pattern recognition principle.Results:The model correctly identified Lonicerae Japonicae Flos and Lonicerae Flos,the established method is reliable,fast and simple,and can be used as the control method for the authenticity of Lonicerae Japonicae Flos.Conclusion:Near-infrared spectroscopy combined with SIMCA pattern recognition method is feasible in the classification and identification of Lonicerae Japonicae Flos and Lonicerae Flos.