高纯度平阳霉素铜螯合物纯化工艺研究

Purification Process of High-purity Pingyangmycin Chelating Copper

本试验采用单分散聚合物微球填料柱色谱法纯化平阳霉素铜螯合物粗品,使其HPLC纯度高于98%。首先采用XR 3SP微球反相色谱纯化,使纯度和单步收率分别提高至50%和85%以上。然后经过POlyRP 30-300反相色谱纯化,纯度和单步收率可分别提高至98%和88%以上,总收率达75%以上。该工艺简单可靠,适于工业化生产。

The crude pingyangmycin(1)chelating copper was purified by column-based chromatography with the prepacked column of monodisperse polymer microspheres,and the HPLC purity of 1 chelating copper reached above 98%.The crude 1 chelating copper was purified sequencely by reversed column-based chromatography with the prepacked column of XR 3SP microspheres and POlyRP 30-300 microspheres.The purify and yield for the first step reached over 50%and 85%,while those for the second step were up to 98%and 88%.The total yield reached above 75%.The process was simple,reliable and suitable for industrial production.