干露胁迫对青蛤呼吸代谢及抗氧化相关酶活力的影响

Effects desiccation on respiratory metabolism and antioxidant enzyme activities of clam Cyclina sinensis

研究了不同时间和温度下干露对青蛤(Cyclina sinensis)失水率、存活率、呼吸代谢相关酶及抗氧化相关酶的影响。结果表明,随着干露时间的增加,青蛤的失水率显著增加,且高温状态下青蛤失水加剧;在3℃和10℃条件下干露48 h未出现死亡现象。在20℃条件下,干露时间超过24 h后,青蛤出现死亡现象,死亡率为3.33%,至48 h死亡率增加至5.33%;随着干露时间的增加,青蛤的呼吸代谢相关酶的活性整体呈现显著下降趋势(P<0.05),10℃条件下青蛤的呼吸代谢相关酶的活性整体高于3℃和20℃;随着干露时间的增加,青蛤的抗氧化相关酶的活性呈现与青蛤呼吸代谢相关酶相似的变化趋势,且不同温度条件下,干露时间达到48 h时,青蛤的抗氧化相关酶活性显著低于对照组(P<0.05)。综上表明青蛤受干露胁迫时具有一定的自我调节能力,但干露时间超过其耐受限度时会对青蛤的生理状态造成影响。

The effect of air-exposure on water loss rate,survival rate and the enzyme activity of the respiratory metabolism-related enzyme and antioxidant-related enzymes of Cyclina sinensis was studied.The result showed that the water loss rate of C.sinensis increased significantly with the increase of air-exposure time,and the water loss of clams increased under high temperature.There was no death at 3℃and 10℃during 48 hours,however,after more than 24 hours,C.sinensis began to die at 20℃,and the mortality rate was 3.33%and 5.33%at 24 hours and 48 hours respectively.The activities of the respiratory metabolism-related enzyme decreased significantly(P<0.05)with the increase of air-exposure time,and the activities of the respiratory metabolism-related enzyme at 10℃was higher than that of clams at 3℃and 20℃.The activities of antioxidant-related enzymes decreased significantly(P<0.05)with the increase of air-exposure time,and the activities of antioxidant-related enzymes were significantly lower than those in the control group(P<0.05)in 48 h at different temperatures.In conclusion,the C.sinensis has self-regulation ability under the stress of air-exposure,however,the physiological state of C.sinensis will be affected when the air-exposure time exceeds its tolerance limit.