神经脱细胞基质凝胶/BMP-2/脱钙骨复合材料的制备及性能

Preparation and properties of acellular nerve matri hydrogel/BMP-2/demineratized bone matrix composite materials

以神经脱细胞基质(NAM)凝胶作为载体,在多孔脱钙骨内表面负载BMP-2,制备新型复合骨修复材料。采用扫描电镜观察材料微观结构,结果表明脱钙骨基质(DBM)复合负载BMP-2的脱细胞基质凝胶后有利于细胞粘附;力学检测结果显示脱钙骨未复合神经脱细胞基质凝胶时,材料的抗压强度为(1.04±0.44)MPa,脱钙骨复合神经脱细胞基质凝胶之后,抗压强度为(1.00±0.30)MPa,材料复合凝胶前后力学性能改变不明显;模拟体内环境,检测不同时间点复合材料中的BMP-2缓释性能,复合材料可缓慢释放BMP-2至少一个月;在材料内部种植MC3TE-E1细胞,共培养14天,CCK-8、碱性磷酸酶检测表明负载BMP-2的神经脱细胞基质凝胶复合脱钙骨材料能够促进细胞增殖和成骨分化。神经脱细胞基质凝胶/BMP-2/钙骨复合材料具有潜在的骨修复应用前景。

A new composite bone repair material was prepared by using a nerve acellular matrix(NAM)gelatin as a carrier to load BMP-2 on the inner surface of porous demineratized bone.The microstructure of the material was observed by scanning electron microscopy.The results showed that the demineratized bone matrix(DBM)combined with the decellularized matrix gelatin of BMP-2 was beneficial to cell adhesion.The mechanical test results showed that the compressive strength of the material was(12.45±0.64)MPa when the composite nerve acellular matrix gelatin was not combined,and the compressive strength was(11.99±0.22)MPa after the composite nerve acellular matrix gelatin.The change was not obvious.Based on the simulation of the in vivo environment,the sustained release of BMP-2 in the composite at different time points was detected.The composite could release BMP-2 slowly for at least one month.MC3TE-E1 cells were cultured in the material for 14 days.CCK-8 and alkaline phosphatase assays showed that BMP-2-loaded nerve acellular matrix gelatin combined with demineratized bone material could promote cell proliferation and osteogenic differentiation.The nerve acellular matrix gelatin/BMP-2/demineratized bone composite had potential application prospects for bone repair.