人TERT基因启动子区生物信息学分析

Bioinformatic Analysis of Human TERT Gene Promoter Region

目的:探讨人端粒酶逆转录酶基因启动子区的序列特征、转录因子及其结合位点。方法:从NCBI数据库中获取人TERT基因组序列及其启动子序列:利用EMBOSS 6. 6. 0、Cp G finder 1. 0和MethPrimer 1. 0软件预测启动子区Cp G岛的位置;利用Patch 1. 0和PROMO 3. 0. 2软件预测可与TERT基因结合的潜在转录因子及结合位点。结果:TERT基因定位于5p15. 33,基因序列全长共41 881 bp,其启动子位于TERT基因5’端上游2 500 bp处,全长2 043bp。TERT基因启动子区可能存在2个Cp G岛和118个转录因子。结论:通过生物信息学软件对h TERT基因启动子进行预测分析,可提高对h TERT基因启动子的研究效率,以便更加深入了解h TERT基因的调控机制及其生物学功能。

Objective: To investigate sequence characteristics,transcription factors and binding sites of human telomerase reverse transcriptase( TERT) gene promoter region. Methods: The sequence of human TERT gene and its promoter sequence were obtained from National Center for Biotechnology Information. The position of CpG island in the promoter region was predicted by EMBOSS 6. 6. 0,CpG finder 1. 0 and MethP rimer 1. 0. Potential transcription factors binding to TERT and their binding sites were predicted by Patch 1. 0 and PROMO 3. 0. 2. Results: The sequence of the TERT gene was located at5 p15. 33,and the full length of the sequence was 41,881 bp. The promoter was located in the 2,500 bp upstream of the 5’end of the TERT gene,and the full length of it was 2,043 bp. There might be two CpG islands and 118 transcription factors in the promoter region of the TERT gene. Conclusion: Predictive analysis of human TERT gene promoter by bioinformatics software can improve the efficiency in studying human TERT gene promoter,so as to understand regulatory mechanisms and biological functions of human TERT gene.