黄精多糖对大强度运动大鼠肾脏损伤的调理作用

Regulatory effect of polysaccharides on kidney injury induced by high-intensity exercise rats

为探讨黄精多糖对大强度运动大鼠肾脏损伤的调理作用,将33只试验大鼠,淘汰6只后,分为安静组、运动组和运动给药组,每组9只。试验中,运动组有1只大鼠死亡。运动给药组小鼠灌服剂量为150 mg·(kg·d)-1黄精多糖溶液,其他2组按质量灌服相应体积的生理盐水。运动组、运动给药组进行为期8周的大强度跑台训练。测定各组大鼠外周血肌酐(SCr)、尿素氮(BUN)含量,肾组织丙二醛(MDA)、一氧化氮(NO)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮合酶(总NOS、iNOS、eNOS)、三磷酸腺苷酶(Na+/K+-ATP、Ca2+/Mg2+-ATP)活性。结果表明:与安静组相比,运动组、运动给药组SCr、BUN、MDA、NO含量及总NOS、iNOS升高, SOD、GSH-Px、Na+/K+-ATP、Ca2+/Mg2+-ATP降低,运动组eNOS降低;与运动组相比,运动给药组SCr、BUN、MDA、NO含量及总NOS、iNOS活性降低, SOD、GSH-Px、Na+/K+-ATP、Ca2+/Mg2+-ATP活性升高。这一研究说明黄精多糖能改善肾小球的滤过功能,提升肾组织抗氧化酶活性,抑制自由基的生成,通过调节iNOS、eNOS活性,减少NO代谢产物对肾组织的毒副作用,提升ATPase活性,维持细胞膜内外Na+、K+、Ca2+、Mg2+正常分布,提示黄精多糖对大强度运动导致的肾脏损伤有一定的正向调理作用。

This paper aims to investigate the effects of polysaccharides on kidney injury in rats with high-intensity exercise. Of 33 experimental rats, 6 rats were eliminated and 27 were divided into sedentary control group, trained group and dosage plus trained group with 9 rats in each group. In the experiment, one rat died in the exercise group. The mice in the dosage plus trained group were given 150 mg·(kg·d)-1 yellow essence polysaccharide solution and the other two groups were given the corresponding volume of normal saline according to the quality. Trained group and dosage plus trained group received 8 weeks of high-intensity treadmill training. The contents of MDA, NO, SOD, GSH-Px and total NOS, iNOS, eNOS, Na+/K+-ATP, Ca2+/Mg2+-ATP in peripheral blood SCr, BUN, kidney tissue of rats in each group were measured. The results showed that compared with sedentary control group, the content of SCr, BUN, MDA, NO, total NOS, iNOS from dosage plus trained group were increased, SOD, GSH-Px, Na+/K+-ATP and Ca2+/Mg2+-ATP were decreased and eNOS in trained group was decreased. Compared with the trained group, the SCr, BUN, MDA, NO content, total NOS, iNOS were decreased and SOD, GSH-Px, Na+/K+-ATP, Ca2+/Mg2+-ATP were increased in the dosage plus trained group. Polysaccharides can improve glomerular filtration function, increase the activity of antioxidant enzymes and inhibit the production of free radicals in renal tissues. Regulating the activity of iNOS, eNOS can reduce the toxic and side effects of NO metabolites on renal tissues and enhance the activity of ATP enzymes and maintain the normal distribution of Na+, K+, Ca2+, Mg2+ in and out of the cell membrane. The results suggest that the polysaccharides has positive conditioning effect on kidney injury induced by high intensity exercise.