高粱醇溶蛋白-卡拉胶复合纳米颗粒负载姜黄色素的特性研究

Characteristic Study Curcumin Loaded by Kafirin-Carrageenan Composite Nanoparticles

本研究以姜黄色素为芯材,高粱醇溶蛋白为壁材,卡拉胶自组装,采用反溶剂法制备负载姜黄色素的中空和实心复合纳米颗粒,并对其理化性质及体外消化特性进行研究。结果表明,制备中空(C-HNP)和实心纳米颗粒(C-SNP)的最佳芯壁比为1∶10,其粒径约为71 nm和135 nm,电位为-37.7 mV和-38.2 mV,包埋率为89.6%和84.1%,负载率为8.01%和7.49%。透射电镜显示两种纳米粒子呈规则的球形形态,C-HNP具有内部中空结构。红外光谱结果显示复合颗粒中酰胺峰Ⅰ和Ⅱ均发生蓝移,卡拉胶和姜黄色素一些吸收峰发生相应偏移,说明它们与蛋白质分子发生相互作用。体外消化模拟实验显示复合纳米颗粒显著地增加了姜黄色素的体外溶解释放率,C-HNP具有更高的控释能力。结果表明,高粱醇溶蛋白是一种适合疏水性分子包埋与控释的生物原材料。

This research was conducted by right of adopting anti-solvent method to prepare hollow and solid composite nanoparticles and conducting the research of its physicochemical properties and in vitro digestive characteristics based on the core materials of curcumine,wall materials of kafirin,assembling of carrageenan.The results showed that the optimal core/wall material ratio was 1∶10.Under this condition,the mean diameter and Zeta potential of the C-HNP and C-SNP were^71 and 135 nm,as well as-37.7mV and-38.2 mV,respectively;the encapsulation efficiencies were 89.6%and 84.1%,and the loading efficiencies were 8.01%and 7.49%,respectively.Transmission electron microscopy showed that both nanoparticles had regular spherical shape,while C-HNP contained internal hollow structure.Fourier-transform infrared spectroscopy indicated that the peaks of amideⅠandⅡin the nanoparticles blue shifted,while the partial absorption peaks of carrageenan and curcumin shifted correspondingly,suggesting the formation of interaction among them.In vitro simulated digestion results showed that the obtained nanoparticles significantly improved the release of curcumin,and C-HNP exhibited higher controlled release capability.The results showed that kafirin is a kind of biological raw material suitable for the encapsulation and controlled release of hydrophobic molecule.