黄芩与葡萄糖酸锌联用对痤疮致病菌的抑菌实验研究

Study on antibacterial activity of combined application of Scutellaria baicalensis and zinc gluconate against pathogenic bacteria of acne

[目的]探究黄芩与葡萄糖酸锌联用对痤疮致病菌是否具有协同抑菌作用。[方法]采用牛津杯法检测黄芩和葡萄糖酸锌单用及联用时对痤疮致病菌的抑菌活性。采用微量二倍稀释法和棋盘法分别测定两者单用及联用时的最低抑菌浓度(MIC),计算部分抑菌浓度指数(FICI)判定药物相互作用。绘制时间-生长曲线进一步考察药物作用特点。[结果]黄芩与葡萄糖酸锌联用后对痤疮致病菌的抑菌活性显著增强,MIC均下降。两者联用对金黄色葡萄球菌的FICI为0.75,表现为部分协同作用,对痤疮丙酸杆菌的FICI为0.25,表现为协同作用。联合用药对痤疮致病菌的抑菌强度更大,持续时间更长。[结论]黄芩联合葡萄糖酸锌对痤疮致病菌具有协同抑菌作用,可为新型抑菌剂的开发奠定基础。

[Objective]To explore whether the combined application of Scutellaria baicalensis and zinc gluconate has a synergistic antibacterial effect on pathogenic bacteria of acne.[Methods]The antibacterial activity of single or combined application of Scutellaria baicalensis and zinc gluconate against pathogenic bacteria of acne were measured by the Oxford Cup method.The minimum inhibitory concentration(MIC)of Scutellaria baicalensis,zinc gluconate and combined application were carried out by the micro-dilution method and checkerboard method,respectively.The Fractional Inhibitory Concentration Index(FICI)was calculated to determine their interaction.Furthermore,the synergistic effect of the optimized combination was evaluated through the time-growth curve.[Results]The antibacterial activity of combined application of Scutellaria baicalensis and zinc gluconate against pathogenic bacteria of acne was significantly stronger than single application.The MIC for combined application were both decline.The FICI of combined application on staphylococcus aureus was 0.75,which showed partial synergistic effect,the FICI of combined application on propionibacterium acne was 0.25,which showed synergistic effect.It has a more sustained antibacterial effect on acne pathogenic bacteria under the combined application.[Conclusion]The combined application of Scutellaria baicalensis and zinc gluconate has significant synergistic antibacterial effect on acne pathogens,which can provide some references for developing new drugs.