酱香型白酒提取物抗幽门螺杆菌代谢产物诱导的血管内皮细胞损伤作用研究

Moutai-Flavor Liquor Extract Isolation and Anti-inflammatory Damage in Human Vascular Endothelial Cells induced by Helicobacter Pylori Metabolites

目的:研究酱香型白酒中生物活性物质(BSML)抗幽门螺杆菌代谢产物(Helicobacter pylori metabolite,HPM)诱发炎性损伤的作用及机制。方法:采用氯仿萃取及真空冻干获得BSML,GC-MS分析其主要成分,HPM诱导人脐静脉内皮细胞(HUVEC-12)建立炎性损伤模型;MTT法检测BSML和HPM对HUVEC-12细胞活性的影响;ELISA测定BSML处理细胞对相关炎症因子白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-6(Interleukin-6,IL-6)、白细胞介素-8(Interleukin-8,IL-8)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)分泌的影响,Western blotting检测BSML刺激细胞对NF-κB,MAPK信号通路的影响。结果:BSML可增强HUVEC-12细胞抗HPM导致的细胞活性降低,可显著降低HPM导致炎性因子IL-6、IL-8、TNF-α的分泌并促进抑炎因子IL-4的表达。Western blotting结果显示BSML可通过调控NF-κB,MAPK等信号通路发挥抗HPM诱发的炎性损伤。结论:酱香型白酒中含有的活性物质具有一定的抗炎活性,可通过抑制炎症因子IL-6、IL-8和TNF-α,促进抑炎因子IL-4的分泌及调控NF-κB、MAPK等信号通路发挥抗炎作用。

Objective:To study the effect and mechanism of BSML in Maotai-flavor liquor on anti-inflammatory injury induced by HPM.Methods:BSML was extracted by chloroform and freeze-dried in vacuum.The main components of BSML were analyzed by GC-MS.human umbilical vein endothelial cells(HUVEC-12)were induced by HPM to establish an inflammatory injury model.MTT method was used to detect the effects of BSML and HPM on HUVEC-12 cell activity.ELISA was used to detect the effects of BSML treated cells on the related inflammatory factors,IL-4 and IL-6,The effects of IL-6,IL-8,TNF-αsecretion and the effects of BSML stimulation on NF-κB,MAPK signaling pathway were detected by Western blotting.Results:BSML could enhance the anti HPM activity of HUVEC-12 cells,decrease the secretion of IL-6,IL-8,TNF-αand promote the expression of IL-4.Western blotting showed that BSML could inhibit HPM induced inflammatory injury by regulating NF-κB,MAPK and other signaling pathways.Conclusion:the active substances contained in Maotai-flavor liquor have certain anti-inflammatory activity,which can play an anti-inflammatory role by inhibiting the secretion of IL-6,IL-8 and TNF-α,promoting the secretion of IL-4 and regulating NF-κB,MAPK and other signal pathways.