c-erb-B2在早期胃癌术后复发预测中的作用

The role of c-erb-B2 in predicting postoperative recurrence of early gastric cancer

目的探讨c-erb-B2在早期胃癌术后复发预测中的作用。方法收集178例早期胃癌术后患者的手术切除石蜡组织标本。所有患者均进行了随访,采用计算机断层扫描(CT)、内窥镜活检、正电子发射断层成像(PET)-CT评估早期胃癌的复发情况。采用免疫组织化学染色(IHC)和荧光原位杂交(FISH)技术分别检测cerb-B2蛋白的表达情况和c-erb-B2基因的扩增状态。结果178例早期胃癌术后患者的复发率为7.87%(14/178)。不同性别、肿瘤浸润深度、淋巴结转移情况的早期胃癌术后患者的复发情况比较,差异均有统计学意义(P﹤0.05);不同年龄、分化情况、手术方式、肿瘤位置、肿瘤分型和肿瘤直径的早期胃癌术后患者的复发情况比较,差异均无统计学意义(P﹥0.05)。178例早期胃癌患者中,18例患者c-erb-B2阳性,包括复发患者12例和未复发患者6例。c-erb-B2阳性早期胃癌患者的复发率明显高于c-erb-B2阴性患者(P﹤0.01)。结论c-erb-B2阳性早期胃癌患者的复发率高,其可作为一种早期胃癌术后复发的预测分子标志物。

Objective To investigate the role of c-erb-B2 in predicting the postoperative recurrence of early gastric cancer(EGC).Method The paraffin embedded tissue specimens from 178 cases with EGC were collected during surgery.All patients were followed up regularly.Computed tomography(CT),endoscopy,and positron emission tomography(PET)-CT were used to evaluate the recurrence of EGC.Immunohistochemistry(IHC)and fluorescence in situ hybridization(FISH)were employed to detect c-erb-B2 protein expression and gene amplification.Result The recurrence rate of EGC was 7.87%(14/178)among the 178 cases with EGC after surgery.Sex,depth of tumor invasion and lymph node metastasis were related to the recurrence of EGC,with statistically significant differences noted(P<0.05);while age,degree of differentiation,surgical approach,tumor site,tumor subtype and tumor diameter were independent of EGC recurrence(P>0.05).Among the 178 cases of EGC,18 were with positive c-erb-B2,including 12 cases of recurrence and 6 without recurrence.Significantly higher recurrence rate was observed in c-erb-B2 positive patients compared to c-erb-B2 negative ones(P<0.01).Conclusion Positive expression of c-erb-B2 is associated with increased recurrence potential for EGC,thus may be employed as a predictive molecular biomarker for the recurrence of EGC.