产KPC型碳青霉烯酶肺炎克雷伯菌的检测及耐药状况研究

Detection and Drug Resistance of KPC-type Carbapenemase Against Klebsiella Pneumoniae

目的本次研究分析产KPC型碳青霉烯酶肺炎克雷伯菌的检测方法以及耐药情况研究。方法本次研究样本选取吉林省人民医院和吉大一院临床分离的耐碳青霉烯酶的肺炎克雷伯菌,菌株选取50例,研究时间从2016年1月1日至2018年9月1日,对所有的耐碳青霉烯酶的肺炎克雷伯菌株采用Hodge试验以及PCR检测,然后再对其进行药敏试验,判断其耐药状况。结果从50例耐碳青霉烯酶的肺炎克雷伯菌株的检测情况上看,在Hodge试验中,其中KPC阳性菌株41例,阳性率为82.0%;在PCR检测中,检测出KPC阳性菌株43例,阳性率为86.0%。从药敏试验上看,其中对于头孢类药物耐药率为100%,对于阿米卡星药物耐药率最低,为40.0%。结论在本院50例耐碳青霉烯酶的肺炎克雷伯菌株当中,发现了KPC阳性菌株,并且该病菌对于不同药物的临床耐药性不同,对于头孢类药物耐药率较高,具体耐药性还需要进一步检测。

Objective This study was conducted to analyze the detection method and drug resistance of KPC-type carbapenemase Klebsiella pneumoniae.Methods The samples from this study were selected from Jilin Provincial people’s hospital and the fi rst Hospital of Jilin University clinical isolates of K.pneumoniae resistant to carbapenemase,and 50 strains were selected.The study time was from January 1,2016 to September 1,2018,for all The K.pneumoniae strain resistant to carbapenemase was tested by Hodge test and PCR,and then tested for drug susceptibility to determine its drug resistance.Results From the detection of 50 cases of K.pneumoniae strains resistant to carbapenemase,41 cases of KPC positive strains were positive in the Hodge test,and the positive rate was 82.0%.In the PCR test,KPC was detected.There were 43 strains with a positive rate of 86.0%.From the susceptibility test,the resistance rate to cephalosporins was 100%,and the resistance rate to amikacin was the lowest,40.0%.Conclusion Among the 50 Klebsiella pneumoniae strains resistant to carbapenemase in our hospital,KPC-positive strains were found,and the resistance to different drugs was different,and the resistance rate to cephalosporins was higher.Specifi c resistance needs further testing.