尿中锰测定的离子液体微萃取-石墨炉原子吸收光谱法

Determination of manganese in human urine by dispersive ionic liquid-liquid microextraction-graphite furnace atomic absorption spectrometry

目的建立离子液体微萃取-石墨炉原子吸收光谱法测定尿锰含量.方法以乙醇为分散剂,8-羟基喹啉为螯合剂,离子液体1-辛基-3-甲基咪唑六氟磷酸盐([Omim][PF6])为萃取剂,萃取尿锰.采用单因素轮换试验确定萃取条件后,评价离子液体微萃取-石墨炉原子吸收光谱法测定尿锰的精密度、准确度和检出限等性能指标.结果该方法测定尿锰线性范围为0.0~1.6μg/L,相关系数为0.992,检出限为0.03μg/L,样品加标回收率为84.90%~96.50%,相对标准偏差为0.36%~1.84%.结论该方法检出限低,灵敏度高,回收率高,可用于职业接触人群及普通人群尿锰的测定.

Objective To establish a method for the determination of manganese in urine with graphite furnace atomic absorption spectrometry(GFAAS)by using ionic liquid microextraction.Methods The ethanol,8-hydroxyquinoline and ionic liquid 1-octyl-3-methyl-imidazolium hexafluorophosphate were used as dispersive solvent,chelating agent and extraction solvent respectively,for the preconcentration of manganese.After the optimal extraction conditions were optimized by single factor rotations,evaluate the performance indicators such as methodological precision,accuracy,and detection limit.Results The linear range of urine manganese was 0.0-1.6μg/L,and the correlation coefficient of standard curve line was 0.992,the detection limit was 0.03μg/L,the recovery of sample spiked was 84.90%-96.50%,and the relative standard deviation was 0.36%-1.84%.Conclusion The method has the advantages of low detection limit,high recovery rate and high sensitivity.It is suitable for the determination of manganese in urine samples from occupational exposure populations and the general population.