迷你岩桐组织培养及植株再生体系建立

Tissue culture studies,and the plant regeneration system of Sinningia hybrida‘Isa’s Murmur’

以迷你岩桐(Sinningia hybrida‘Isa’s Murmur’)带柄叶片为外植体,探讨了外植体的灭菌方法、不同基本培养基以及外源激素(6-BA、NAA、IBA)对愈伤组织和不定芽的诱导及增殖、生根等的影响,成功建立了迷你岩桐组织培养植株再生体系。结果表明:迷你岩桐带柄叶片在75%乙醇灭菌10 s,0.1%升汞灭菌300 s时,污染率较低(15.56%)而启动率较高(85.56%)。在MS培养基中添加2.0 mg·L^-16-BA和0.1 mg·L^-1 NAA时,愈伤组织诱导率最高(91.11%)。在1/2 MS培养基中添加2.0 mg·L^-16-BA和0.05 mg·L^-1 NAA时,不定芽诱导率为88.89%。在MS培养基中同时添加2.0 mg·L^-16-BA和0.1 mg·L^-1 NAA时,不定芽增殖效果最好,增殖倍数为2.38。1/2 MS+0.10 mg·L^-1 IBA培养基有利于生根,生根率为87.78%。腐殖土∶泥炭土∶河沙以体积比为1∶2∶2的混合基质为移栽基质时,瓶苗移栽成活率可达96.67%。本研究结果可为迷你岩桐的遗传转化体系的构建及规模化生产提供一定的理论基础和技术支撑。

The effects of various disinfection methods,the basic medium and exogenous hormone ratios on callus inductions,adventitious bud inductions,proliferation,and rooting were investigated using leaves from Sinningia hybrida‘Isa’s Murmur’as explants.We established a regeneration system via tissue culture of S..The results showed that when leaf tissue of S.was sterilized with 75%ethanol for 10 s followed by 0.1%mercuric chloride for 300 s,the pollution rate was the lowest(15.56%).MS medium with 2.0 mg·L^-16-BA and 0.1 mg·L^-1 NAA exhibited the highest callus inductions rate(91.11%).The 50%MS medium with 2.0 mg·L^-16-BA and 0.05 mg·L^-1 NAA was optimal for adventitious bud inductions,with a adventitious bud induction rate of 88.89%.The optimal medium for adventitious bud proliferation was MS containing 2.0 mg·L^-16-BA and 0.1 mg·L^-1 NAA.The proliferation rate was 2.38.The optimal rooting medium was 50%MS medium containing 0.1 mg·L^-1 IBA,with a rooting rate of 87.78%.The survival rate was above 96.67%when plantlets were moved into mixed matrices containing 1 humus:2 peat soil:2 river sand.The results of these studies can provide a basis for genetic transformation and large-scale propagation of Sinningia hybrida‘Isa’s Murmur’.