基于脑肠肽胃促生长素水平探讨制萎扶胃丸对萎缩性胃炎大鼠作用的实验研究

Study on intervention mechanism of Zhiwei Fuwei Pillson Rats with Atrophic gastritis Based on Ghrelin Level of Cerebrointestinal Peptide

目的通过制备萎缩性胃炎大鼠模型,观察制萎扶胃丸(ZWFW)对模型大鼠胃促生长素(ghrelin)表达水平的调控,探讨其防治慢性萎缩性胃炎(chronic atrophic gastritis,CAG)的可能作用机理。方法采用随机数字表法将70只Wistar大鼠分为正常对照组(空白组)15只,造模组55只,以2%水杨酸钠乙醇液(醇浓度为30%)与10 mmol/L脱氧胆酸钠交替灌胃,0.05%氨水自由饮用,结合饥饱失常建立CAG大鼠模型。于造模结束从两组随机抽取大鼠各5只,HE染色观察大鼠胃窦组织病理改变,以验证造模。于造模结束后,将造模组大鼠随机分为模型组、ZWFW低、中、高剂量组及摩罗丹组,每组10只。药物干预组分别用配置液以1 mL/100 g灌胃,空白组及模型组用生理盐水以1 mL/100 g灌胃,6组均每日2次,给药4周。药物干预结束后观察各组大鼠的宏观表征及病理组织学变化;记录各组大鼠的进食量与体质量,ELISA法检测各组大鼠血清ghrelin含量变化。结果较之模型组,ZWFW各剂量组及摩罗丹组大鼠进食量、体质量及血清ghrelin含量增加,差异均有统计学意义(P<0.01,P<0.05);而ZWFW中、高剂量组大鼠血清ghrelin含量变化与摩罗丹组比较,差异有统计学意义(P<0.05),低剂量组与摩罗丹组比较,血清ghrelin变化无统计学意义(P>0.05)。结论ZWFW可增加CAG大鼠进食量及体质量,改善胃黏膜病理状态,可能与ZWFW上调血清ghrelin水平有关,也是ZWFW防治CAG胃黏膜萎缩的作用机理之一。

Objective The effects of Zhiwei Fuwei Pills(ZWFW)on the level of ghrelin in the stomach of rats with atrophic gastritis were observed,and the possible mechanism of its prevention and treatment of CAG was discussed.Method:Seventy Wistar rats were randomly divided into normal control group(control group,n=15)and model group(n=55).Sodium salicylate ethanol solution(30%alcohol concentration)and 10 mmol/L sodium deoxycholate were perfused alternately into stomach with 0.05%ammonia water for drinking freely.CAG rat model was established in combination with hunger and satiety disorder.At the end of model making,5 rats were randomly selected from each group,and the pathological changes of gastric antrum were observed by HE staining to verify whether the model was successful or not.The rats in the model group were randomly divided into low,middle and high dose groups and Moro Dan group with 10 rats in each group.The medication intervention groups were given the corresponding solution with 1 mL/100 g by gavage.Rats in the blank group and the model group were given normal saline daily with 1 mL/100 g for intragastric administration.The six groups were all treated twice a day,lasting for 4 weeks.After treatment,the macroscopical features and histopathological changes of the rats were observed,the food intake and body weight of the rats were recorded,and the changes of serum ghrelin content in each group were detected by ELISA method.Results Compared with the model group,the rats in the ZWFW group and the Moro Dan group increased their body weight and food intake(P<0.01),and the difference was statistically significant(P<0.05)in each dose group of ZWFW compared with that in the Moro Dan group(P<0.05),but there was no significant difference between the two groups(P<0.05).Compared with the model group,the serum ghrelin content in the ZWFW group and the Moro Dan group was significantly higher than that in the model group(P<0.01,P<0.05),and the ghrelin content in the high dose group was higher than that in the Moro Dan group.The difference was statistically significant(P<0.05),but there was no significant difference between the low dose group and Moro Dan group(P>0.05).Conclusion ZWFW can increase the intake and body weight of CAG rats and improve the pathological state of gastric mucosa,which may be related to the up-regulation of serum ghrelin level by ZWFW,which is also one of the mechanisms of ZWFW in preventing and treating CAG.