肠道病毒A组71型热力和常见3种化学消毒剂灭活效果的研究

Inactivation effect of heat and common three chemical disinfectants on enterovirus A 71

目的评价热力灭活和3种常见化学消毒剂对肠道病毒A组71型(enterovirus A71,EV-A71)的灭活效果,为肠道病毒二级生物安全实验室实验活动后的消毒工作提供基础数据。方法分别在不同温度和不同消毒剂浓度下,按照不同的作用时间处理一定滴度的EV-A71病毒,将灭活处理后的病毒接种于96孔细胞培养板中,逐日观察并记录细胞病变效应,最后计算病毒处理液的病毒滴度。结果EV-A71病毒在56℃加热灭活30 min后可被有效灭活(灭活对数值>6),在65℃加热灭活30 min、70℃加热灭活10 min后,无细胞病变效应发生;3种化学消毒剂在一定浓度和作用时间下可以有效灭活EV-A71病毒。结论EV-A71病毒的灭活效果与热力灭活温度、化学消毒剂浓度和处理作用时间等因素有关,对EV-A71的灭活曲线进行研究,有利于在不同灭活环境下灭活条件的选择。

Objective To evaluate the inactivation effect of heat inactivation and three common chemical disinfectants on enterovirus group A 71(EV-A71),provide basic data for disinfection work after laboratory activities in enterovirus secondary biosafety laboratory.Methods EV-A71 virus was treated at different temperatures and different disinfectant concentrations according to different time.The inactivated virus was inoculated into 96-well cell culture plates,and the cytopathic effect was observed and recorded.Finally,the virus titer of the virus treatment solution was calculated.Results After inactivation at 56°C for 30 min,the inactivation logarithm of EV-A71 was more than 6,and inactivation at 65°C for 30 min,70°C for 10 min,no infection was observed.Three chemical disinfectants could effectively inactivate a certain titer of EV-A71 at a certain concentration and treatment time.Conclusions The inactivation effect of EV-A71 virus is related to factors such as thermal inactivation temperature,chemical disinfectant concentration,and treatment time.Studying the inactivation curve of EV-A71 is beneficial to the selection of inactivation conditions in different inactivation environments.