绵羊FRZB基因InDel检测及其与生长性状的关联分析

Detection of InDel of FRZB Gene and Its Association Analysis with Growth Traits in Sheep

本研究以卷曲相关同源蛋白(FRZB)基因作为候选基因,以同羊、小尾寒羊、兰州大尾羊和湖羊4个品种共计582只绵羊个体为试验材料,利用琼脂糖凝胶电泳结合DNA测序技术对FRZB基因的InDel进行筛查,旨在寻找与4个品种绵羊生长性状相关的InDel位点。结果表明,绵羊FRZB基因第1内含子上检测到一段14 bp的InDel突变;FRZB基因内含子区InDel突变位点在同羊、小尾寒羊、兰州大尾羊和湖羊群体中均存在II、ID、DD 3种基因型;关联分析表明,FRZB基因中检测到的InDel突变对同羊生长性状有显著影响(P<0.05),其中II基因型的体长、背高、荐高、距骨宽度、尾长和尾宽在同羊群体中显著优于DD基因型,可以作为候选分子标记用于同羊分子标记辅助选择。这些结果提示,绵羊FRZB基因第1内含子上一个14 bp的插入突变可显著影响同羊生长性状,可以作为绵羊育种中生长性状的潜在分子标记。

In this study,FRZB gene was selected as a candidate gene,582 sheep were selected as experimental materials from four breeds of Tong sheep,Small-tailed Han sheep,Lanzhou Big-tailed sheep and Hu sheep.DNA pool sequencing combined with agarose gel electrophoresis was used to screen the InDel of FRZB gene in order to find the sites of InDel related to the growth traits of four sheep breeds.The results showed that a 14 bp InDel mutation was detected in the first intron of FRZB gene in sheep;The InDel mutation site in the intron region of FRZB gene had three genotypes(II,ID and DD)in Tong Sheep,Small-tailed Han sheep,Lanzhou Big-tailed sheep and Hu sheep populations;Association analysis showed that the InDel mutation detected in FRZB gene had significant effects on the growth traits in Tong sheep.The body length,back height,hip height,talus width,tail length and tail width of II genotype were significantly superior to DD genotype in Tong sheep(P<0.05),it could be used as a candidate molecular marker for the molecular marker-assisted selection of Tong sheep.These results suggested that a novel 14 bp insertion mutation in the first intron of FRZB gene in sheep could be significantly affecting the growth trait of Tong sheep,and could be used as a potential molecular marker for growth traits in sheep breeding.