拟康宁木霉Hailin菌株对马尾松幼苗的促生和防病作用

Effect of Trichoderma koningiopsis on the growth promotion and disease control of Pinus massoniana seedlings

采用菌株形态学和r DNA-ITS序列比对分析的方法进行了Hailin菌株的鉴定,采用平皿对峙法研究了Hailin菌株对尖孢镰孢菌(Fusarium oxysporum)的抑菌效果,测定了接种尖孢镰孢菌和Hailin菌株后对马尾松(Pinus massoniana Lamb)幼苗的发病率和生长指标的影响。经形态学、分子鉴定和进化树分析,菌株Hailin为拟康宁木霉(Trichoderma koningiopsis)。Hailin菌株与尖孢镰刀菌平皿对峙培养5d后,对其抑制率为77.78%;蘸根针刺微伤处理接种Hailin菌株30 d后,对马尾松幼苗立枯病的防治率为58.3%;接种Hailin菌株与不接种的马尾松幼苗相比,其株高、湿重、根长分别提高了3.86%、78.74%和13.2%。Hailin菌株抑制尖孢镰孢菌的作用机制包括竞争作用、重寄生作用以及通过产生代谢产物抑制病原菌的生长。其中,Hailin菌株通过非挥发性代谢产物和挥发性代谢产物对尖孢镰孢菌菌丝生长的抑制率分别为97.80%和77.80%。

The identification of Hailin strain was carried out by strain morp hology and r DNA-ITS sequence alignment analysis.The inhibitory effect of Hailin strain on F.oxysporum was studied by the method of plate confrontation.By measuring the incidence and growth index of the seedlings inoculated with F.oxysporum and Hailin strain,the growth promotion and disease control effect of Hailin strain on P.massoniana seedlings were studied.The Hailin strain was identified as T.koningiopsis through morphological and molecular identification as well as phylogenetic tree analysis.Its inhibition rate against F.oxysporum was 77.78%after confront culture for five days.After 30 days of seedling inoculation with Hailin strain by root dipping with slight injury,the preventive effect against P.massoniana seedling damping-off was58.2%.Compared with the non-inoculated P.massoniana seedlings,the height,wet weight,and root length of the seedlings inoculated with Hailin strain have increased by 3.86%,78.74%,and 13.2%respectively.The mechanism underneath the inhibitory effect of Hailin strain against F.oxysporum includes competition,hyperparasitism,and the production of metabolites that inhibit the growth of F.oxysporum.The inhibition rates of Hailin strain non-volatile metabolites and volatile metabolites on the growth of F.oxysporum mycelium were 97.80%and 77.80%,respectively.