口腔鳞状细胞癌中PD-L1的表达与P16、HPV感染以及淋巴结转移关系分析

Relationship between expression of PD-L1 and P16, HPV infection and lymph node metastasis in oral squamous cell carcinoma

目的观察口腔鳞状细胞癌中程序性死亡分子-L1配体(PD-L1)的表达与乳头瘤病毒-16 (P16)、人乳头瘤病毒(HPV)感染以及淋巴结转移关系,初步探讨PD-L1在口腔鳞状细胞癌病情发展中的作用。方法以本院2017年6月至2018年6月期间收集到的口腔鳞状细胞癌组织蜡块为研究标本,行采用免疫组化染色法检测组织标本PD-L1、P16及HPV表达情况。观察PD-L1、P16、HPV的表达情况,并采用Spearman等级相关性分析法分析PD-L1与P16、HPV感染以及淋巴结转移关系的相关性。结果 51例口腔鳞状细胞癌组织中,PD-L1呈阳性表达44例(86.27%),PD-L1呈阴性表达7例(13.72%),P16呈阳性表达22例(43.14%),P16呈阴性表达29例(56.86%)。口腔鳞状细胞癌组织中的PD-L1表达与P16表达呈负相关(rs=-0.819,P=0.000),与淋巴结转移分期之间呈负相关(rs=-0.736,P=0.000),与HPV感染呈正相关(rs=0.653,P=0.000)。结论 PD-L1在口腔鳞状细胞癌组织中呈高度表达,且与口腔鳞状细胞癌病情发展标志物P16、HPV密切相关,同时在淋巴结转移中也具有促进作用,因此,深入研究PD-L1在口腔鳞状细胞癌病情发展的作用,有助于口腔鳞状细胞癌免疫疗法的完善。

Objective To investigate the relationship between the expression of programmed death molecule-L1 ligand(PDL1) and papillomavirus-16(P16), human papillomavirus(HPV) infection and lymph node metastasis in oral squamous cell carcinoma(OSCC). Methods The expression of PD-L1, P16 and HPV in oral squamous cell carcinoma(OSCC) tissue samples collected from June 2017 to June 2018 were detected by immunohistochemical staining. The expressions of PD-L1, P16 and HPV were observed, and the correlation between PD-L1, P16, HPV infection and lymph node metastasis was analyzed by Spearman hierarchical correlation analysis. Results PD-L1 was positive in 44 cases(86.27%), PD-L1 was negative in 7 cases(13.72%), P16 was positive in 22 cases(43.14%) and P16 was negative in 29 cases(56.86%). The expression of PD-L1 in oral squamous cell carcinoma was negatively correlated with the expression of P16(rs=-0.819, P=0.000), negatively correlated with lymph node metastasis stage(rs=-0.736, P=0.000), and positively correlated with HPV infection(rs=0.653, P=0.000). Conclusion PD-L1 is highly expressed in oral squamous cell carcinoma, and is closely related to the development of oral squamous cell carcinoma markers P16 and HPV. It also promotes lymph node metastasis. Therefore, further study on the role of PD-L1 in the development of oral squamous cell carcinoma is helpful to the immunity of oral squamous cell carcinoma. The improvement of treatment.