高脂饲喂结合颈总动脉球囊损伤制作胰岛素抵抗兔动脉粥样硬化模型

Establishment of Atherosclerosis Model Induced by Feeding High-fat Diet Plus Carotid Artery Balloon Injury in Rabbits with Insulin Resistance

目的探索制作快速、稳定可靠的兔动脉粥样硬化(AS)模型的方法.方法采用高脂饲料喂养结合动脉内膜球囊损伤的方法建立兔AS模型.选择健康雄性新西兰白兔20只,体质量2.0~2.5 kg,实施颈总动脉内膜球囊损伤术后予以高脂饲料喂养,于饲喂前和饲喂12周后,取耳缘静脉血测定总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、空腹血糖(FBG)及空腹胰岛素(FSI),计算胰岛素敏感指数(ISI)和稳态模型-胰岛素抵抗(HOMA-IR);在肉眼及光学显微镜下观察颈总动脉内膜形态特征.结果高脂饲喂12周后,可见体质量、TC、TG和LDL-C较饲喂前均显著升高(P<0.01);FBG无明显变化(P=0.550),FSI及HOMA-IR较饲喂前显著升高,HDL-C、ISI较饲喂前显著降低(P<0.01);颈总动脉内壁肉眼可见典型动脉粥样斑块,光学显微镜下见内膜明显增厚,形成明显的粥样斑块及纤维帽结构.结论高脂饲喂可导致兔胰岛素抵抗,联合动脉内球囊损伤法可以短期内成功制作兔AS模型.

Objectives To identify a quick,stable and reliable method for establishing atherosclerosis(AS)model in rabbit.Methods The atherosclerosis rabbit models were established by feeding high-fat diet plus carotid artery balloon injury.twenty healthy New Zealand white male rabbits weighting 2.0-2.5 kg were injured by carotid artery balloon and fed with high fat diet.Before and after 12 weeks feeding,blood from jugular vein were collected to detect total cholesterol(TC),triglyeride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),fasting blood glucose(FBG),fasting serum insulin(FSI),insulin sensitivity index(ISI)and homeostasis model assessment insulin resistance(HOMA-IR).Total carotid artery endometrium morphology was observed with naked eye and microscope.Results After feeding high fat diet for 12 weeks,the body weight,TC,TG,LDL-C of the rabbits were significantly higher than pre-treatment(P<0.01).The difference of FBG was not statistically significant compared with pre-treatment(P=0.550).FSI and HOMA-IR were significantly higher than pre-treatment(P<0.01).HDL-C and ISI were significantly lower than pre-treatment(P<0.01).The typical atherosclerosis mottling in common carotid artery lumen under macrography was visible and intima thickness increased significantly.Plaque and structure of fibrous cap had formed.Conclusions The typical carotid atherosclerosis lesion can be successfully induced by high fat diet combining with balloon injury in carotid artery.On the other hand,high fat diet can induce insulin resistance model.