润燥明目汤对急性高眼压大鼠视网膜及其组织Caspase-3、Bcl-2、Bax表达的影响

Effect of Runzao Mingmu Decoction on Expression of Caspase-3,Bcl-2 and Bax in Retina and Its Tissue of Rats with Acute High Intraocular Pressure

目的探讨润燥明目汤保护急性高眼压大鼠视神经膜细胞的可能机制.方法将60只SD雄性大鼠随机分为6组,根据处理方式的不同分为空白组、模型组、阳性药物组、润燥明目汤低剂量组、中剂量组及高剂量组.检测并记录各组大鼠眼压变化及视网膜组织中细胞凋亡相关因子的表达.结果(1)造模后即刻,空白组大鼠眼压无明显变化,其他组大鼠眼压显著升高(P<0.05).用药后8周,单纯模型组、低剂量组、中剂量组的眼压无明显差异(P>0.05),但高剂量组大鼠眼压明显低于阳性药物组(P<0.05);(2)空白组大鼠的超氧化物歧化酶(SOD)水平最高,丙二醛(MDA)、一氧化氮(NO)、谷氨酸(Glu)、Ca^2+水平最低(P<0.05).与单纯模型组相比,各给药剂量组及阳性药物组的相关指标差异均存在统计学意义(P<0.05);且与阳性药物组相比,高剂量干预组大鼠视网膜组织中NO、Glu水平呈逐渐下降趋势(P<0.05);(3)与单纯模型组相比,各组大鼠视网膜神经节细胞中的Bcl-2平均积分吸光度逐渐升高(P<0.05);而Bax、Caspase-3的平均积分吸光度逐渐下降,呈现出剂量依赖性(P<0.05);(4)与单纯模型组相比,各组大鼠视网膜神经节细胞中的Bcl-2 mRNA相对表达量逐渐升高,Bax mRNA、Caspase-3 mRNA的相对表达量逐渐下降,均呈现出剂量依赖性(P<0.05);高剂量干预组大鼠视神经组织中Bcl-2 mRNA、Bax mRNA表达均优于阳性药物组(P<0.05).结论润燥明目汤对急性高眼压大鼠视神经有明显的保护作用,其机制可能与抑制Caspase-3/Bcl-2/Bax信号通路活性有关.

Objective To explore the possible mechanism of Runzao Mingmu Decoction in protecting optic nerve membrane cells in rats with acute high intraocular pressure.Methods Sixty male Sprague-Dawley rats were randomly divided into 6 groups.According to different treatment methods,they were divided into blank group,model group,positive drug group,Runzao Mingmu Decoction low dose group,medium dose group and high dose group.The changes of intraocular pressure and the expression of apoptosis-related factors in retinal tissue were detected and recorded in each group.Results(1)Immediately after modeling,there was no significant change in the blank group,and the intraocular pressure of the other groups was significantly increased(P<0.05).There was no significant difference in intraocular pressure between the model group,the low-dose group and the middle-dose group at 8 weeks after treatment(P>0.05),but the intraocular pressure of the high-dose group was significantly lower than that of the positive drug group(P<0.05).(2)The superoxide dismutase(SOD)level was the highest in the blank group,and the levels of malondialdehyde(MDA),nitric oxide(NO),glutamic acid(Glu)and Ca^2+were the lowest(P<0.05).Compared with the simple model group,there were significant differences between the drug-administered dose group and the positive drug group(P<0.05).Compared with the positive drug group,the levels of NO and Glu in the retinal tissue of the high-dose intervention group were Gradually decreasing trend,the difference was statistically significant(P<0.05).(3)The average integral optical density of Bcl-2 in rat retinal ganglion cells increased gradually with the increase of treatment dose,but there was no significant difference(P>0.05),while the average integral optical density of Bax and Caspase-3 decreased gradually,showing a dose-dependent(P<0.05).(4)Compared with the simple model group,the relative expression of Bcl-2 mRNA in the retinal ganglion cells of each group increased gradually,and the relative expression of Bax mRNA and Caspase-3 mRNA decreased gradually,all of which showed a dose-dependent difference.Statistically significant(P<0.05);the expression of Bcl-2 mRNA and Bax mRNA in the optic nerve tissue of the high dose intervention group was better than that of the positive drug group(P<0.05).Conclusion Runzao Mingmu Decoction has obvious protective effect on optic nerve in rats with acute high intraocular pressure.The mechanism may be related to the inhibition of Caspase-3/Bcl-2/Bax signaling pathway activity.