摘要
目的观察针刺结合顽痰汤对卒中相关性肺炎模型大鼠的治疗效果。方法动物分组:选取成年雄性大鼠120只,体质量230~280 g,鼠龄12周,从中随机抽取30只分为空白对照组、假手术组,每组15只,剩余大鼠造模完成后,用抽签法随机分为模型组、针刺组、中药低剂量组,中药高剂量组,针刺+中药低剂量组,针刺+中药高剂量组,共8组。空白对照组不做任何处理;假手术组只暴露右侧颈总、颈内和颈外动脉,不造成栓塞;模型组是通过线栓造成脑缺血再灌注而致肺炎模型;在模型建立后,针刺治疗组对模型使用针刺治疗;中药低、高剂量组分别使用低、高剂量中药治疗;针刺+中药低、高剂量治疗组分别使用针刺+低、高剂量中药治疗(其中所有组别中中药为顽痰汤加减)。结果治疗后,与空白对照组比较,模型组肺组织WBC,MPO活性均升高,差异有统计学意义(P <0. 05),假手术组各项指标差异无统计学意义(P> 0. 05);与模型组比较,中药低剂量组肺组织WBC,MPO活性降低不明显,差异无统计学意义(P> 0. 05),余各治疗组的各项指标均降低,差异有统计学意义(P <0. 05);针刺+中药高剂量组分别与中药高剂量组、针刺组进行组间比较,肺组织WBC,MPO活性差异有统计学意义(P <0. 05)。结论针刺结合顽痰汤治疗大鼠卒中相关性肺炎有一定的疗效,且针刺+中药高剂量组治疗卒中相关性肺炎疗效明显。
Objective To observe the therapeutic effect of acupuncture combined with Wantan Decoction on stroke-associated pneumonia model rats.Methods A total of 120 adult male rats weighing 230-280 g and 12 weeks old were selected and divided into 8 groups,15 in each group.While 30 rats among them were randomly selected and put into blank control group and sham operation group.The remaining rats were randomly divided into model group,acupuncture group,Wantan Decoctionlow-dose group,Wantan Decoctionhigh-dose group,acupuncture+Wantan Decoctionlow-dose group,acupuncture+Wantan Decoction high-dose group.The blank control group was without any treatment.The sham operation group was only exposed the right common,internal and external carotid arteries without embolism.The model group were rats with pneumonia induced by cerebral ischemia and after restoration of blood flow with thread embolism.After the model was established,the acupuncture treatment group used acupuncture,the low and high dose groups of traditional Chinese medicine used low and high dose Chinese medicine treatment respectively(in all groups,the traditional Chinese medicine was Wantan Decoction).Results After treatment,compared with the blank control group,the activity of WBC and MPO in the lung tissue of the model group increasedwith statistical significance(P<0.05),but there was no significant difference in the indexes of the sham operation group(P<0.05).Compared with the model group,the activity of WBC and MPO in the lung tissue of the low dose group of Chinese medicine was not significantly decreased(P>0.05)and the other indexes of the treatment groups were all decreased.The difference was statistically significant(P<0.05).The WBC and MPO activities of lung tissue in the high dose group of acupuncture and traditional Chinese medicine were significantly different from those in the high dose group of traditional Chinese medicine and the acupuncture group(P<0.05).Conclusion Acupuncture combined with Wantan Decoction has certain curative effect on stroke-associated pneumonia in rats.In addition,the curative effect was obvious for treatment with both acupuncture and high-dose traditional Chinese medicine.
作者
王颖
吴明健
吴海洋
WANG Ying;WU Mingjian;WU Haiyang(Neurology Department,The Second Affiliated Hospital of Anhui University of Chinese Medicine,Anhui 230000,Hefei,China;Anhui University of Chinese Medicine,Anhui 230000,Hefei,China)
出处
《辽宁中医杂志》
CAS
2020年第2期199-202,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
安徽省教育厅自然科学研究项目(KJ2018A0294)。
关键词
卒中相关性肺炎
针刺结合顽痰汤治疗
实验研究
stroke-associated pneumonia
treatment with combination of acupuncture and Wantan Decoction
experimental study