PHACTR1基因多态性与皖北地区汉族人群冠心病的相关性

Correlation between gene polymorphisms of PHACTR1 and coronary heart disease in Han population in north of Anhui Province

目的探讨PHACTR1基因rs2026458、rs9349379位点多态性与皖北地区汉族人群冠状动脉粥样硬化性心脏病(冠心病,CHD)的相关性。方法纳入2017年12月至2018年12月于蚌埠医学院第一附属医院心血管科住院的冠心病患者为CHD组(n=93),选取同期在此医院经心电图和病史排除冠心病的体检者为对照组(n=104)。采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测两组人员rs2026458及rs9349379位点的基因型和等位基因分布。结果两组人员rsl761667位点及rs10499859位点的等位基因频率及基因型相比,差异均无统计学意义(P>0.05)。与对照组相比,CHD组年龄较大,体重、高血压、2型糖尿病、吸烟史、HbA1c、TG、LDL等指标显著高于对照组,HDL显著低于对照组,差异均有统计学意义(P<0.05)。Logistic回归分析显示,低密度脂蛋白胆固醇是冠心病发生的独立危险因素(P<0.05)。结论皖北地区汉族人群PHACTR1基因rs2026458和rs9349379位点多态性可能与冠心病的发生无关。

Objective To discuss the correlation between the gene polymorphisms of PHACTR1 at rs2026458 site and rs9349379 site and coronary heart disease(CHD)in Han population in north of Anhui Province.Methods CHD patients were chosen into CHD group(n=93)from Department of Cardiovascular Medicine in the First Affiliated Hospital of Bengbu Medical College from Dec.2017 to Dec.2018,and at the same time,the controls without CHD excluded by ECG and medical history were chosen into control group(n=104).The genotypes and allele distribution of rs2026458 site and rs9349379 site were detected by using polymerase chain reaction-based restriction fragment length polymorphism(PCR-RFLP)in 2 groups.Results The difference in allele frequency and genotypes of rs2026458 site and rs9349379 site had no statistical significance between 2 groups(P>0.05).In CHD group,the patients were older with higher weight,percentages of patients with hypertension,type 2 diabetes mellitus(T2DM)and smoking history were higher,levels of HbA1c,TG and LDL were significantly higher,and HDL level was significantly lower compared with control group(P<0.05).The results of Logistic regression analysis showed that LDL was an independent risk factor of CHD(P<0.05).Conclusion The gene polymorphisms of PHACTR1 at rs2026458 site and rs9349379 site may not be related to CHD incidence in Han population in north of Anhui Province.