丹参川芎嗪注射液对肺栓塞大鼠P-选择素及组织因子的影响

Effect of salvia miltiorrhiza and ligustrazine injection on P-selectin and tissue factor in rats of pulmonary embolism

目的:通过观察肺栓塞大鼠用药后血中P-选择素(CD62P)及组织因子(TF)的变化,研究丹参川芎嗪注射液、低分子肝素及两者联合应用对肺栓塞大鼠的影响。方法:健康雄性Wistar大鼠80只,随机分为7 h组及1周组,每组又分为对照组、栓塞组、丹参川芎嗪组、低分子肝素组、联合用药组,每小组8只大鼠。将大鼠分离右侧颈静脉,对照组注射生理盐水,其余各组注入自制栓子(25±3)个(约0.5 mm/个)。各组在第7小时及第7天采血,检测TF及CD62P浓度,并观察肺组织。结果:栓塞后大鼠可见喘憋、发绀、心率加快等,肺组织可见表面凹凸不平,膨胀不均,散在灰白色梗死区域。栓塞组与对照组相比,血浆中TF、CD62P浓度均显著增高( P值均<0.05);3个用药组与栓塞组相比,TF、CD62P的浓度均有不同程度的降低,其中治疗1周的联合用药组与栓塞组比降低最为明显。 结论:使用颈静脉注入异体血栓法可成功制备理想的肺栓塞大鼠模型;丹参川芎嗪与低分子肝素联合应用可显著降低肺栓塞大鼠CD62P及TF的水平。

Objective To study the effects of salvia miltiorrhiza and ligustrazine injection,low molecular weight heparin and their combination on pulmonary embolism rats by observing the changes of P-selectin(CD62P)and tissue factor(TF)in the blood of rats of pulmonary embolism.Methods 80 healthy male Wistar rats were randomly divided into 7 h and 7 d groups.Each group was divided into control group,embolism group,salviae miltiorrhiza and ligustrazine group,low molecular weight heparin group,and combined drug group,each group with eight rats.The rats were separated from the right jugular vein,the control group was injected with saline,and the remaining groups were injected with homemade emboli(n=25±3,d=0.5 mm).Each group collected blood at the 7th hour and the 7th day,TF and CD62P concentrations were detected,and lung tissue was observed.Results After embolization,rats had panting,cyanosis,accelerated heart rate,etc.The surface of lung tissue was rugged,uneven expansion,scattered in gray-white infarction areas.Compared with the control group,the concentrations of TF and CD62P in plasma of the embolism group increased significantly(all P<0.05).Compared with the embolism group,the concentrations of TF and CD62P of the therapy groups decreased to different degrees,and the combined group after therapy for one week was most significant.Conclusions The ideal model of pulmonary embolism can be prepared by jugular vein injection allothrombosis.The levels of TF and CD62P in pulmonary embolism rats could be significantly reduced by the combination of salvia miltiorrhiza and ligustrazine and low molecular weight heparin.