高效液相色谱法测定调味茶饮料中甜味剂和防腐剂

Determination of sweeteners and preservatives in seasoning tea-based drinks by high performance liquid chromatography

建立测定调味茶饮料中常用的2种甜味剂(糖精钠、阿斯巴甜)和3种防腐剂(苯甲酸、山梨酸、脱氢乙酸)的高效液相色谱分析方法。使用Diamonsil C18柱(5μm,250 mm×4.6 mm),以甲醇-0.02 mol/L醋酸铵水溶液(含0.4 mmol/L甲酸)为流动相进行梯度洗脱,样品在50℃下超声提取30 min后用紫外可见可变波长检测器检测。结果表明,5种目标物质在20 min内基线分离,标准曲线的相关系数r>0.999,平均回收率为83.0%~108.0%,日内精密度RSD<3.6%,日间精密度RSD<4.4%。该方法可用于多种调味茶饮料中这5种目标物质的分析。

Develop a method for the determination of 2 kinds of sweeteners(saccharin sodium,aspartame)and 3 kinds of preservatives(benzoic acid,sorbic acid,dehydroacetic acid)in seasoning tea-based drinks by high performance liquid chromatography(HPLC).A Diamonsil C18 column(5μm,250 mm×4.6 mm)was used with methanol-0.02 mol/L ammonium acetate(containing 0.4 mmol/L formic acid)solution as the mobile phase under the condition of gradient elution.Samples were extracted with water in an 50℃ultrasonic bath for 30 min,and then they were analysed by VWD.Results show that the targets are baseline separated in 20 min.All correlation coef cients(r)are more than 0.999,with the average recoveries range from 83.0%to 108.0%,intra-day precision(RSD)values below 3.6%and inter-day precision(RSD)values below 4.4%.The method can be used for analyzing the targets in seasoning tea-based drinks.