卡其百合的不定芽诱导及植株再生

Adventitious shoot induction and plant regeneration of lily varieties khaki

采用卡其百合(Lillium spp.var.Khaki)的种球为外植体,以MS为基本培养基,添加不同浓度配比的植物生长调节剂,对外植体进行不定芽的诱导、生根及植株再生。对6-BA、NAA、GA3、IBA及活性炭等成分的添加浓度及配比对卡其百合不定芽诱导及生根的影响进行了正交试验。结果表明,不定芽诱导的最佳培养基配方为MS培养基附加2.0 mg/L 6-BA、0.10 mg/L NAA和0.5 mg/L GA3,经验证其不定芽诱导率可达83.3%,芽较为粗壮,色嫩绿,褐化现象很少;不定芽生根的最佳培养基配方为1/2 MS附加0.1 mg/L NAA和0.1 mg/L IBA,经验证其生根率可达73.3%,每个不定芽生根数目较多,根较为粗壮,状态良好。

With the bulbs of Lillium spp.var.Khaki as explants,MS medium plus different concentrations of plant growth regulators were used to inducing adventitious shoots and roots for plant regeneration.In this process,orthogonal experimental methods were used to analysis the impact of the concentrations and proportions of 6-BA,NAA,GA,IBA and active carbon to shoots and roots inducement of Lillium spp.var.Khaki.The results showed that the optimal medium for shoots inducement was MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0.5 mg/L GA3.After retesting,it was proved that the ratio of shoots inducement was 83.3%and the shoots were strong,fresh green and less browning.The optimal medium for rooting of adventitious shoots was 1/2MS+0.1 mg/L NAA+0.1 mg/L IBA and the ratio of rooting was 73.3%.The number of roots for each adventitious shoot was higher.The roots were strong and in good condition.