工程大肠杆菌高效转化外源脂肪酸生成羟基脂肪酸的研究

Study on high efficient transformation of exogenous fatty acids into hydroxyfatty acids by engineering Escherichia coli

细胞色素P450单加氧酶(P450BM3)能特异性催化脂肪酸ω碳位羟基化反应生成羟基脂肪酸,在前期构建携带单加氧酶基因P450BM3工程大肠杆菌(Escherichia coli)转化脂肪酸的基础上,为解决外源脂肪酸在羟基化过程中的消耗和转运问题,构建了脂酰基辅酶A合成酶基因(fadD)缺失和外膜蛋白编码基因(fadL)过表达的工程菌株。摇瓶条件下羟基十二酸产量达到748.8 mg/L,转化率为93.6%。

Cytochrome P450BM3 can hydroxylates fatty acids into hydroxyfatty acids(HFAs)at theω-positions with high entioselectivity.Based on the construction of Escherichia coli carrying P450BM3 gene to transform fatty acids.As an effort to solve inhibit fatty acidβ-oxidation and increase outer membrane fatty acids transporter,the lipoacyl-coa synthase gene(fadD)deletion and outer membrane proteins encode gene(fadL)overexpression strains were built.Under shake-flask conditions,this recombinant strains produced 748.8 mg/L hydroxy dodecanoic acid,meanwhile the conversion rate was 93.6%.