有抗菌能力的牙周引导组织再生膜的制备研究

Preparation of Antibacterial Periodontal Guided Tissue Regeneration Membrane

目的:在聚乳酸-乙醇酸共聚物[poly(lactic-co-glycolic acid),PLGA]表面接枝2-甲基丙烯酰氧乙基磷酸胆碱(2-methacryloyloxyethyl phosphorylcholine,2-MPC),制备一种具有抗菌能力的牙周引导组织再生膜。方法:用紫外光接枝聚合法将2-MPC接枝于PLGA表面,采用傅立叶变换红外光谱验证接枝的成功,并用扫描电镜观察样品改性前后的表面形貌。以大肠杆菌以及金黄色葡萄球菌为实验菌株,在已接枝的和未接枝的样品表面接种1×106 CFU/mL浓度的细菌悬液,37℃孵育4 h,梯度脱水后扫描电镜观察。并且在材料表面接种小鼠成骨细胞系MC3T3-E1培养12 h,进行活细胞染色观察样品表面细胞形态。结果:红外和扫描电镜结果证明2-MPC成功接枝于PLGA膜表面,抗菌实验结果显示接枝后的材料抗大肠杆菌以及金黄色葡萄球菌粘附能力显著提高,并且体外细胞实验结果证明,表面接枝2-MPC在有效提高材料抗菌能力的同时,2-MPC对材料的生物相容性并没有明显的抑制作用。结论:在PLGA表面接枝2-MPC可以获得一种具有优良抗菌性能的牙周组织引导再生膜材料。

Objective:To prepare antibacterial periodontal guided tissue regeneration membrane by grafting 2-methacryloyloxyethyl phosphorylcholine(2-MPC)onto PLGA film.Methods:2-MPC was grafted to PLGA surface by ultraviolet induced grafting polymerization.Fourier-transform infrared(FTIR)was used to confirm the success of grafting.The surface morphology of the samples was observed by scanning electron microscopy(SEM).The samples were fixed with 2.5%glutaraldehyde for 10 h after incubating with E.coli and S.aureus at a concentration of 1×106 CFU/mL(in PBS)for 4 h.And then the samples were dehydrated in graded series of ethanol solutions,dried at room temperature,coated with gold,and examined by SEM.In addition,mouse osteoblastic cell line MC3T3-E1 was seeded on the surface of the material and cultured for 12 h.The living cell staining was conducted to observe the cell morphology on the surface of the samples.Results:FTIR and SEM showed that 2-MPC was successfully grafted on the PLGA membrane.The grafted materials possessed excellent resistance to E.coli and S.aureus,and MPC did not significantly influence the biocompatibility of the materials in vitro.Conclusion:PLGA grafted with 2-MPC could be used as a kind of periodontal tissue guided regeneration membrane which has antibacterial property and biocompatibility.