GO-SiO2-CMC修饰玻碳电极制备生物传感器对VB6的痕量检测研究

Trace Detection of VB6 by Biosensor Prepared by GO-SiO2-CMC Modified Glassy Carbon Electrode

成功制备了氧化石墨烯(GO)和SiO2/CMC的复合膜(GO-SiO2-CMC),将其修饰固体玻碳电极后构成电化学传感器用于维生素B6(VB6)的痕量检测.利用扫描电子显微镜和电化学技术分别研究该复合膜的微观形貌和电化学性能.结果表明,修饰电极对VB6表现出较强的电化学敏感性,对VB6的检测具有较高的灵敏度(31.5μAmM^-1cm^-2)、较低的检测限(10-7M)和较小的表面阻抗值(252Ω).GO-SiO2-CMC对VB6的检测,提供了一种新型的简单高效的VB6检测技术.

A novel platform based on reduced graphene oxide and SiO2/CMC hybrid membranes(GO-SiO2-CMC)was successfully constructed for the fabrication of biosensor.The morphologies and electrochemistry of the nanocomposite film were investigated by using scanning electron microscopy and electrochemical techniques including electrochemical impedance spectroscopy and cyclic voltammetry(CV),respectively.The results suggested that the modified electrode showed enhanced sensitivity towards the oxidation of Vitamin B6(VB6),e.g.,high detection sensitivity(31.5μAmM^-1cm^-2),a low limit of detection(10-7M),a small Ret value(252Ω)and a good stability.This might be attributed to large surface-to-volume ratio and high conductivity of graphene,which could promote direct electron transfer between the surface of electrodes and VB6.These above results offered a simple,sensitive and flexible method for quantitative detection of VB6 through the technology of electrochemical.