灵芝孢子粉总三萜的提取优化及其体外生物活性

Optimization of total triterpenoids extraction from Ganoderma lucidum spore powder and evaluations of their biological capacities in vitro

对灵芝孢子粉总三萜的提取过程进行优化,以得率为指标,通过单因素试验、Plackett-Burman试验筛选出影响灵芝孢子粉总三萜得率的显著因素,利用响应面设计优化灵芝孢子粉总三萜的提取条件。对于提取出的灵芝孢子粉总三萜,评价其清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基和ABTS^+自由基的方法,并采用CCK-8比色法就其抗人肝癌细胞HepG-2增殖的活性进行研究。结果表明,最佳提取条件为乙醇体积分数80%,固液比1∶23,超声功率540 W,提取温度40℃,提取时间60 min。此优化条件下提取的灵芝孢子粉总三萜具有较好的抗氧化能力,清除ABTS^+的能力强于清除DPPH,对人肝癌细胞HepG-2亦具有一定的抑制作用。

To optimize the ultrasonic extraction technology of total triterpenoids from Ganoderma lucidum spore powder, and investigate its antioxidant and antitumor activity in vitro, the extraction method was optimized to achieve high yield. The significant influencing factors were firstly screened by Plackett-Burman(PB) design on the basis of single factor experiment, and then were further optimized by Box-Behnken. Antioxidant activity of total triterpenoids in vitro was evaluated by DPPH and ABTS^+ free radical scavenging activity assay and the effect of total triterpenoids on the human hepatoma HepG-2 cells was detected by CCK-8. The results showed that the optimal extraction conditions were as follows: ethanol volume fraction of 80%, solid-liquid ratio of 1∶23, ultrasonic power of 540 W, ultrasonic temperature of 40 ℃, ultrasonic time of 60 min. The bioactivity studies showed that the total triterpenoids extracted from Ganoderma lucidum spore powder had better ability to scavenge ABTS^+ than DPPH free radicals, and had anti-proliferation activity against human hepatoma cell(HepG-2).