乙醇-硫酸铵双水相萃取紫色红曲菌发酵液中的红曲色素及其稳定性分析

Extraction and Stability of Monascus Pigments from Fermentation Broth of Monascus purpureus YY1-3 Using Ethanol/Ammonium Sulfate Aqueous Two-Phase System

建立一种采取乙醇-硫酸铵双水相体系,从紫色红曲菌YY1-3菌株发酵液中简单、高效萃取红曲色素(Monascus pigments,MPs)的方法,利用薄层色谱(thin layer chromatography,TLC)分析提取液的色素成分,并考察了该菌株产红曲色素的稳定性。结果表明:当萃取体系中添加质量分数16%的乙醇和28%的硫酸铵时,MPs的分配系数与回收率达到最大,分别为171.91和98.13%;当上相萃取液-乙醇体积比为1∶0.5时,可有效分离回收上相中的硫酸铵;TLC分析结果表明,萃取液中有6种主要色素组分,其中以红曲红和红曲黄色素为主;色素稳定性实验结果表明,在避光、pH 4~8、25~65℃以及常规金属离子和食品添加剂溶液中,该菌株所产的MPs具有良好的稳定性。

Monascus pigments(MPs)were extracted from the fermentation broth of Monascus purpureus YY1-3 in a simple and efficient way using an ethanol/ammonium sulfate aqueous two-phase system(ATPS).Thin layer chromatography(TLC)was used to analyze the composition of the MPs and their stability was also tested.The results showed that when the ATPS was composed of 16%(m/m)ethanol and 28%(m/m)ammonium sulfate,the maximum partition coefficient and recovery rate of MPs of 171.91 and 98.13%were obtained respectively.When the volume proportion of the top phase extraction solution-ethanol was 1:0.5,ammonium sulfate could be effectively removed from it.TLC showed that the extract was composed of six components,mainly Monascus red and yellow pigments.The MPs were stable in darkness,pH 4–8 and 25–65℃,as well as in the presence of common metal ions and food additives.