摘要
采用氢核磁共振(1H nuclear magnetic resonance,1H NMR)代谢组学技术对酸枣仁、理枣仁以及不同比例的掺伪粉末进行对比,结合多元统计分析建立一种快速鉴别酸枣仁粉末及其掺伪品的方法。主成分分析显示掺伪比例较低的样品与酸枣仁有部分的重叠。偏最小二乘法-判别分析(partial least squares-discriminant analysis,PLS-DA)显示,当掺伪比例不低于40%时,酸枣仁粉和掺伪品沿t[1]轴完全分开。在此基础上,采用PLS对酸枣仁及其不同掺伪比例粉末样品训练集数据进行回归分析,并建立掺伪判别模型。1H NMR与PLS相结合的模型预测结果准确性较高,可实现酸枣仁中理枣仁掺假的快速鉴别。该方法为酸枣仁及含酸枣仁的保健食品、复方制剂质量评价提供了一条新途径。
In the present study,the chemical composition of pure powders of Ziziphi spinosae Semen(ZSS)and Ziziphi mauritianae Semen(ZMS)and their mixtures in different proportions was compared by 1H nuclear magnetic resonance(1H NMR)-based metabolomic fingerprinting.Then the obtained spectral data were analyzed by multivariate analysis to develop a new method for rapid detection of ZSS adulteration.Principal component analysis(PCA)performed on the 1H NMR data revealed that only a few samples at low adulteration ratios were overlapped with ZSS.In addition,the data were also analyzed by partial least squares-discriminant analysis(PLS-DA).A clear separation was seen between ZSS and 40%adulterated samples.Then a PLS model was established based on the 1H NMR data and was applied to detect adulteration in ZSS falsified by intentional addition of different concentrations of ZMS,and a quantitative calibration model was established.The PLS regression approach provided a highly reliable and accurate prediction model.The results demonstrated that 1H NMR spectroscopy coupled with PLS is a fast and effective technique for checking adulteration in ZSS.Meanwhile,the method provides a potential tool for quality control of ZSS.
作者
申晨曦
杜晨晖
李震宇
李爱平
秦雪梅
闫艳
SHEN Chenxi;DU Chenhui;LI Zhenyu;LI Aiping;QIN Xuemei;YAN Yan(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;School of Traditional Chinese Materia Medica,Shanxi University of Traditional Chinese Medicine,Taiyuan 030619,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第8期275-281,共7页
Food Science
基金
国家自然科学基金青年科学基金项目(81603289,81603251)
山西省研究生联合培养基地人才培养项目(2017JD01)
山西省科技攻关计划-振东专项(2016ZD0105)
山西省科学技术厅-地产中药功效物质研究与利用山西省重点实验室项目(201605D111004)
山西大学引进人才事业发展经费项目(226545006)
山西省研究生教育创新项目。
关键词
酸枣仁
理枣仁
掺伪
氢核磁共振
代谢组学技术
偏最小二乘法
Ziziphi spinosae Semen
Ziziphi mauritianae Semen
adulterants
1H nuclear magnetic resonance
metabolomics
partial least squares